Protein Info for EX28DRAFT_2508 in Enterobacter asburiae PDN3

Annotation: Beta-galactosidase/beta-glucuronidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1029 PF02837: Glyco_hydro_2_N" amino acids 56 to 220 (165 residues), 213.7 bits, see alignment E=4.1e-67 PF00703: Glyco_hydro_2" amino acids 222 to 335 (114 residues), 49.8 bits, see alignment E=1.4e-16 PF02836: Glyco_hydro_2_C" amino acids 337 to 629 (293 residues), 393.1 bits, see alignment E=2e-121 PF16353: LacZ_4" amino acids 641 to 728 (88 residues), 72.7 bits, see alignment E=6.1e-24 PF02929: Bgal_small_N" amino acids 758 to 1024 (267 residues), 204.5 bits, see alignment E=5.7e-64

Best Hits

Swiss-Prot: 90% identical to BGAL2_ENTCL: Beta-galactosidase 2 (lacZ) from Enterobacter cloacae

KEGG orthology group: K01190, beta-galactosidase [EC: 3.2.1.23] (inferred from 65% identity to cko:CKO_02825)

Predicted SEED Role

"Beta-galactosidase (EC 3.2.1.23)" in subsystem Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Lactose utilization (EC 3.2.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1029 amino acids)

>EX28DRAFT_2508 Beta-galactosidase/beta-glucuronidase (Enterobacter asburiae PDN3)
MSTTSPLTLSAILARRDWENSGVTQWNRLAAHAPLHSWRNESSAREDAGSASRHVLNGLW
RFSYFTAPERVPQSWVTEDCADAVAMPVPSNWQMQGFDTPIYTNVTYPIPVNPPFVPQEN
PTGCYSLTFEVDDAWLQSGQTRIIFDGVNSAFHLWCNGQWMGYSQDSRLPAEFDLSGALR
PGQNRLAVMVLRWCDGSYLEDQDMWRMSGIFRDVTLLHKPETQIADYHVVTDLNAELDRA
LLKVDVTLAGAGFADCEVAFTLWRNGEKCASVSRRPGSAIVDERGSWAERLTAAIPVDQP
ALWSAEKPELYRLTIALLNPQGEVLEVEACDVGFRRVDISNGQLELNGKPLLIRGVNRHE
HHPENGQVMDEATMRRDIEIMKQHSFNAVRCSHYPNHPLWYRLCDRYGLYVVDEANIETH
GMVPMSRLADDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWLKTT
DPTRPVQYEGGGANTAATDIVCPMYARVDRDQPFPAVPKWSIKKWIGMPDETRPLILCEY
AHAMGNSFGGFAKYWEAFRSHPRLQGGFVWDWVDQALTKRDDDGNTFWAYGGDFGDKPND
RQFCLNGLVFPDRTPHPALYEAQRAQQFFTFTRVSTSPLVIEVQSGYLFRHTDNEVLNWT
VARDGEVLAAGEVTLAMAPEGNQRLEIDLPALKAGPGEVWLNVEVRQPRATPWSPADHRC
AWEQWPLPAPLFIAPPAPAGEPPVLTQSDLHLEIVHRQQRWQFDRATGNLSQWWRNGVET
LLSPLTDNVSRAPLDNDIGVSEATQIDPNAWVERWKAAGMYDLTSRVLHCEAQQHAGDVV
VTTQHVLEYRGKALFMSRKVWRVDDRGVLHGDIQIDVASDIPEPARVGLSVHLAETPKNV
HWLGLGPHENYPDRKLAAQQGRWTLPLEAMHTPYIFPTENGLRCDTRELVVGTHQLNGRF
HFSVSRYSQQQLRETTHHHLLREEPGCWLNLDAFHMGVGGDDSWSPSVSPEFILQTRQLR
YTFSWQQNP