Protein Info for EX28DRAFT_2496 in Enterobacter asburiae PDN3

Annotation: The (Largely Gram-negative Bacterial) Hydrophobe/Amphiphile Efflux-1 (HAE1) Family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1048 transmembrane" amino acids 10 to 30 (21 residues), see Phobius details amino acids 340 to 359 (20 residues), see Phobius details amino acids 366 to 388 (23 residues), see Phobius details amino acids 394 to 413 (20 residues), see Phobius details amino acids 438 to 460 (23 residues), see Phobius details amino acids 470 to 493 (24 residues), see Phobius details amino acids 541 to 558 (18 residues), see Phobius details amino acids 873 to 891 (19 residues), see Phobius details amino acids 898 to 918 (21 residues), see Phobius details amino acids 924 to 947 (24 residues), see Phobius details amino acids 974 to 994 (21 residues), see Phobius details amino acids 1006 to 1028 (23 residues), see Phobius details TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 1 to 1045 (1045 residues), 1898.1 bits, see alignment E=0 PF00873: ACR_tran" amino acids 1 to 1029 (1029 residues), 1461 bits, see alignment E=0 PF03176: MMPL" amino acids 330 to 496 (167 residues), 27.4 bits, see alignment E=2.4e-10

Best Hits

Swiss-Prot: 92% identical to ACRB_ECOLI: Multidrug efflux pump subunit AcrB (acrB) from Escherichia coli (strain K12)

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 70% identity to abo:ABO_0964)

MetaCyc: 92% identical to multidrug efflux pump RND permease AcrB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN0-592

Predicted SEED Role

"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1048 amino acids)

>EX28DRAFT_2496 The (Largely Gram-negative Bacterial) Hydrophobe/Amphiphile Efflux-1 (HAE1) Family (Enterobacter asburiae PDN3)
MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVTISATYPGADAKTVQDT
VTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFQSGTDADIAQVQVQNKLQLAMPLLPQ
EVQQQGVSVEKSSSSFLMVVGVINTNGTMTQEDISDYVGANMKDAISRTSGVGDVQLFGS
QYAMRIWMDPNKLNNFQLTPVDVISAIKAQNAQVAAGQLGGTPPVKGQQLNASIIAQTRL
TSADEFSKILLKVNQDGSQVRLRDVAKVELGGENYDIIAKFNGKPASGLGIKLATGANAL
DTATAIRAELKKMEPFFPSGLKIVYPYDTTPFVKISIHEVVKTLVEAIILVFLVMYLFLQ
NFRATLIPTIAVPVVLLGTFAILSIFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVM
AEEGLPPKEATRKSMGQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMAL
SVLVALILTPALCATMLKPIQKGGHGEHKGFFGWFNRMFDKSTHHYTDSVGNILRSTGRY
LLLYIIIVVGMAFLFVRLPSSFLPDEDQGVFLTMAQLPAGASQERTQKVLDEVTDYYLTK
EKDNVESVFAVNGFGFAGRGQNTGIAFVSLKDWSERPGEKNKVEAITGRAMGTFSQIKDA
MVFAFNLPAIVELGTATGFDFQLIDQGGLGHEKLTQARNQLFGEVAKHPDLLVGVRPNGL
EDTPQYKIDIDQEKAQALGVSISDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAQYRM
LPNDINSWFVRGSNGQMVPFSAFSTSRWEYGSPRLERYNGLPSMEILGQAAPGRSTGEAM
NLMEELASKLPAGIGYDWTGMSYQERLSGNQAPALYAISLIVVFLCLAALYESWSIPFSV
MLVVPLGVIGALLAATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGLI
EATLEAVRMRLRPILMTSLAFILGVLPLVISSGAGSGAQNAVGTGVMGGMVTATVLAIFF
VPVFFVVVRRRFSRKNEDVEHSHSVEPH