Protein Info for EX28DRAFT_2454 in Enterobacter asburiae PDN3

Annotation: cysteinyl-tRNA synthetase (EC 6.1.1.16)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 461 TIGR00435: cysteine--tRNA ligase" amino acids 2 to 460 (459 residues), 651.5 bits, see alignment E=4.7e-200 PF01406: tRNA-synt_1e" amino acids 15 to 313 (299 residues), 498.7 bits, see alignment E=1.7e-153 PF09334: tRNA-synt_1g" amino acids 36 to 136 (101 residues), 25.4 bits, see alignment E=1.5e-09 amino acids 249 to 311 (63 residues), 27.8 bits, see alignment E=2.8e-10 PF00133: tRNA-synt_1" amino acids 222 to 304 (83 residues), 24.1 bits, see alignment E=3.7e-09 PF09190: DALR_2" amino acids 341 to 402 (62 residues), 87.1 bits, see alignment E=2.2e-28

Best Hits

Swiss-Prot: 93% identical to SYC_SALAR: Cysteine--tRNA ligase (cysS) from Salmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980)

KEGG orthology group: K01883, cysteinyl-tRNA synthetase [EC: 6.1.1.16] (inferred from 98% identity to enc:ECL_01272)

MetaCyc: 92% identical to cysteine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Cysteine--tRNA ligase. [EC: 6.1.1.16]

Predicted SEED Role

"Cysteinyl-tRNA synthetase (EC 6.1.1.16)" in subsystem Conserved gene cluster possibly involved in RNA metabolism (EC 6.1.1.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (461 amino acids)

>EX28DRAFT_2454 cysteinyl-tRNA synthetase (EC 6.1.1.16) (Enterobacter asburiae PDN3)
MLKIFNTMTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTFVAFDVVSRYLRFLGYT
LKYVRNITDIDDKIIKRANENGESFVALVDRMIVEMHKDFDALNIQRPDSEPRATHHIHE
IIEITEKLIARGHAYVADNGDVMFSVPTDPTYGSLSRQDLDQLQAGARVDVVDVKRNPMD
FVLWKMSKEGEPSWPSPWGEGRPGWHIECSAMNCKQLGKHFDIHGGGSDLMFPHHENEIA
QSTCAHGGEYVNYWMHSGMVMVDREKMSKSLGNFFTVRDVLKYYDAETVRYFLMSGHYRS
QLNYSEENLKQARSALERLYTALRGTDRSVPAAGGEAFEARFVEVMNDDFNTPEAYSVLF
DMAREVNRLKSEDMAAANALASHLRKLSSVLGLLEQEPDAFLQSGAQADDGEVAEIEALI
KARLEARQAKDWAAADAARNRLTEMGIILEDGPQGTTWRRK