Protein Info for EX28DRAFT_2445 in Enterobacter asburiae PDN3

Annotation: P pilus assembly protein, porin PapC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 853 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF13954: PapC_N" amino acids 29 to 177 (149 residues), 165.3 bits, see alignment E=1.4e-52 PF00577: Usher" amino acids 188 to 765 (578 residues), 727.9 bits, see alignment E=1.5e-222 PF13953: PapC_C" amino acids 774 to 838 (65 residues), 74.5 bits, see alignment 6.9e-25

Best Hits

Swiss-Prot: 74% identical to FIMD_SALTY: Outer membrane usher protein FimD (fimD) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K07347, outer membrane usher protein (inferred from 93% identity to enc:ECL_01281)

Predicted SEED Role

"Outer membrane usher protein SfmD"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (853 amino acids)

>EX28DRAFT_2445 P pilus assembly protein, porin PapC (Enterobacter asburiae PDN3)
MKNHQRRYCPVALALMAALWPQAGWSESYFNPAFLSDDAASVADLSRFEQGHQQAPGVYR
VDIWRNDEFIGTQDVHFEQAENTPPVAGGLSACITRAMLDRFGVNVAAFPELAKVQGDTC
VPLTTAIPGSETAFNFASQRLDVSLPQVALQNSARGYIPPEQWDEGIPAALVNYSFTGNR
GSEDDSYYLNLQSGLNYGAWRLRNNGAWRYTQNNGQRHSEWQNIGTWAQSTVIPLKSELV
LGDSNTGNDVFDSMGFRGGRLFSSDSMYPDSLQGYAPTVRGIARTPAKVVVRQNGYVIYQ
SYVQPGAFAITDLNPTSSSGDLEVTVEEKDGTQQRYTVPYSTVPLLQREGRWKYDLVAGD
YRSGNSDQDTPFFTQGTLVTGLANGYTLYGGTQLASRYTAVAVGAGKNLGDWGAVSLDIT
HARSQLADDSKHEGQSLRFLYAKSLNGFGTNFQLLGYRYSTKGFYTLDDVAWRSMEGYQY
ADSQNDNDVPDVQSYHNLTWNKKGRFQLNVSQSLGDYGSVYISGSEQTYWGTDETNLWYQ
LGYAGGVKGINYSVSWSWNKSVGIDGTDRIASFNVSVPFSLFTRQGYRRDSAIDRAYATA
SASRNSDGDTSWQTGVSGTLLEDRNLNYSVTQGHASTNGSSGSASANWQATYGTLGVGYN
YSRDQHDLNWQLSGGVVGHADGVTFSQPLGDTNVLIKAPGASGVSIENQTGVKTDWRGYA
VMPYATVYRYNRVALDTNTMSNNTDIENNVSSVVPTKGALVRASFDTRIGVRALLTVMRG
NQPVPFGAVVRETKSGVTSMVGDDGQIYLSGLPLEGELLIQWGDGAQSQCRAPYSLSEQS
LQQAITLKGISCD