Protein Info for EX28DRAFT_2417 in Enterobacter asburiae PDN3
Annotation: carboxylate-amine ligase, YbdK family
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 88% identical to GCS2_ENT38: Putative glutamate--cysteine ligase 2 (Ent638_1085) from Enterobacter sp. (strain 638)
KEGG orthology group: K06048, carboxylate-amine ligase [EC: 6.3.-.-] (inferred from 97% identity to enc:ECL_03148)MetaCyc: 76% identical to putative glutamate--cysteine ligase 2 (Escherichia coli K-12 substr. MG1655)
Glutamate--cysteine ligase. [EC: 6.3.2.2]
Predicted SEED Role
"FIG00732033: hypothetical protein"
MetaCyc Pathways
- γ-glutamyl cycle (5/6 steps found)
- glutathione biosynthesis (2/2 steps found)
- ophthalmate biosynthesis (2/3 steps found)
- homoglutathione biosynthesis (1/2 steps found)
- ergothioneine biosynthesis I (bacteria) (3/7 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.3.2.2
Use Curated BLAST to search for 6.3.-.- or 6.3.2.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (373 amino acids)
>EX28DRAFT_2417 carboxylate-amine ligase, YbdK family (Enterobacter asburiae PDN3) MPLPDFKSSEPFTLGIELELQVVNPPGYDLSQDSSALIAAVKDDIKGGEVKHDITESMLE IATGVCQNIDQAAAQFSVMQQSILRAAAEQHIQICGGGTHPFQKWQRQEVCDDERYNVTL ERFGYLILQATVFGQHVHVGCRTGDDAIYLLHGLSRFVPHFIALAAASPYMQGTDTKFSS SRLNIFSGFPDNGQMPWVNSWQEFEGLFRRLSSTSMIDSIKDLHWDIRPSPHFGTVEVRV MDTPLTLGHAINIAGLIQATSHWLLTARPYKHQEKDFLLYRFNRFQACRYGLEGILTDVH TGEHKTVAEDIAWLLEQVAPSAEKLGATSAINEIALLLKQGKSEAQRMREFIADGGSLIS LVQKHCELWATSP