Protein Info for EX28DRAFT_2380 in Enterobacter asburiae PDN3

Annotation: amino acid adenylation domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1285 PF00668: Condensation" amino acids 4 to 437 (434 residues), 302.8 bits, see alignment E=1e-93 PF00501: AMP-binding" amino acids 457 to 807 (351 residues), 302.7 bits, see alignment E=8.5e-94 TIGR01733: amino acid adenylation domain" amino acids 477 to 880 (404 residues), 423.5 bits, see alignment E=3.9e-131 PF13193: AMP-binding_C" amino acids 877 to 945 (69 residues), 25.5 bits, see alignment 5.2e-09 PF00550: PP-binding" amino acids 972 to 1035 (64 residues), 48.6 bits, see alignment 2e-16 PF00975: Thioesterase" amino acids 1062 to 1276 (215 residues), 170.2 bits, see alignment E=2.1e-53

Best Hits

Swiss-Prot: 77% identical to ENTF_ECOLI: Enterobactin synthase component F (entF) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 79% identity to eae:EAE_13615)

MetaCyc: 77% identical to apo-serine activating enzyme (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Enterobactin synthetase component F, serine activating enzyme (EC 2.7.7.-)" in subsystem Siderophore Enterobactin (EC 2.7.7.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.-

Use Curated BLAST to search for 2.7.7.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1285 amino acids)

>EX28DRAFT_2380 amino acid adenylation domain (Enterobacter asburiae PDN3)
MNRLPLVAAQPGIWMAEQLSSLPNAWSVAHYTELKGDIDAPLLAKAIAEGMTQADTLRMR
FAEENGEVWQWVDDTYVLPEPSIVRVTSHDAAVALMEADLNQNLRVESGQPLAFHQLIQV
GESHWYWYQRYHHLVVDGFSFPAITRQIAAIYAAWKKGEPTPASPFTPFAEVVDEYQRYR
DSEAYARDGAFWAEQRRQLPSPVSLSSAPLPGRAATTDILRLKITADGRAFSQLAQAAGQ
AQRTDQALALVALWLGRLTGRLDYAAGFIFMRRMGSAALTATGPVLNVLPLGVNINPQES
LPELALRLANQLKKMRRHQRYDAEQIVRDSGRAAGDEALFGPVLNVKVFDYQLDIDGVEA
ITHTLATGPVNDLELALFPDEQGGLSIEILANKQRYDEATLSRHVARLNAMLMQFAANPD
LRCGDVETVSEQEYQQLARINDTGLALGSTTLADLVAEQASKTPDAPALADAHIELSYRQ
MREQVVALANLLRARGVKPGDSVAVALPRSVFLTLALHGIVEAGAAWLPLDTGYPDDRLR
MMLEDAKPSLLITTDEQLPRFSDLSITAFSYNTLLPAAGAEPLRLATPEQTAYIIFTSGS
TGRPKGVMVGHSAIVNRLKWMQDHYPLDATDVVAQKTPCSFDVSVWEFWWPFIAGAKLVM
AEPDAHRDPQAMQSFFARYGVTTTHFVPSMLAAFVASLTPENAACCKTLKQVFCSGEALP
TELCREWEQLTQAPLHNLYGPTEAAVDVSWYPAFGPELAAVEGNSVPIGFPVWNTGLRIL
DAMMRPVPFGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDN
GAVEYLGRSDDQLKIRGQRIELGEIDRAMLSLPDVAQAVAHACVFNQAAATGGDARQLVG
YVVSESGLPLDREALLESLKAQLPPHMVPVVLLQISALPLSANGKLDRKALPLPELTSKT
SGRAPETETEAAVAQAFAALLGCEVNDIEADFFALGGHSLLAMRLAAQLSRAFERRVTPG
QIMVASTVSKLSALLDSQMSDEQAQRLGYETILPLRESNGPTLFCFHPASGFAWQFSVLA
RYLSPRWSIVGIQSPRPDGPMQQRADLDGVIEHHLATLRQQQPQGPYYLFGYSLGGTLAQ
GIAARLREQGEEVAFLGLLDTWPPETQNWAEKEANGLDPEVLAEIERERQAFIAAQQGQG
SSELFNAIEGNYADAVRLLTTAHSARFDGKATLFVAERTRTMDPQVAWAPWVAELEVYSQ
DCAHVDIISPQAFEKIGPVLREILG