Protein Info for EX28DRAFT_2371 in Enterobacter asburiae PDN3

Annotation: 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 PF00106: adh_short" amino acids 8 to 187 (180 residues), 146.5 bits, see alignment E=1.1e-46 TIGR04316: 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase" amino acids 10 to 249 (240 residues), 344 bits, see alignment E=2.7e-107 PF13561: adh_short_C2" amino acids 14 to 247 (234 residues), 163.8 bits, see alignment E=7.8e-52 PF08659: KR" amino acids 52 to 157 (106 residues), 35 bits, see alignment E=2e-12

Best Hits

Swiss-Prot: 87% identical to ENTA_ECOLI: 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (entA) from Escherichia coli (strain K12)

KEGG orthology group: K00216, 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase [EC: 1.3.1.28] (inferred from 92% identity to ent:Ent638_1128)

MetaCyc: 87% identical to 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (Escherichia coli K-12 substr. MG1655)
2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase. [EC: 1.3.1.28]

Predicted SEED Role

"2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (EC 1.3.1.28) [enterobactin] siderophore" in subsystem Siderophore Enterobactin (EC 1.3.1.28)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.1.28

Use Curated BLAST to search for 1.3.1.28

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (250 amino acids)

>EX28DRAFT_2371 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (Enterobacter asburiae PDN3)
MALDFTGKTVWVTGAGKGIGYATALAFVEAGAQVTGFDLAFPLGDYPFATETLDVANAAQ
VHEVCGCALASLERLDVLVNAAGILRMGATDQLSQKDWQQTFAVNVGGAFNLFQQTMGQF
RRQQGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALTVGLELAGSGVRCNLVSPGSTD
TDMQRILWKSDDAEQQRIRGFGEQFKLGIPLGKIARPQEIASTVLFLASDAASHITLQDI
VVDGGSTLGA