Protein Info for EX28DRAFT_2359 in Enterobacter asburiae PDN3

Annotation: 5'-methylthioribose kinase (EC 2.7.1.100)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 92% identical to MTNK_ENT38: Methylthioribose kinase (mtnK) from Enterobacter sp. (strain 638)

KEGG orthology group: K00899, 5-methylthioribose kinase [EC: 2.7.1.100] (inferred from 93% identity to enc:ECL_03091)

MetaCyc: 87% identical to S-methyl-5-thioribose kinase (Klebsiella pneumoniae)
S-methyl-5-thioribose kinase. [EC: 2.7.1.100]

Predicted SEED Role

"5-methylthioribose kinase (EC 2.7.1.100)" in subsystem Methionine Salvage (EC 2.7.1.100)

MetaCyc Pathways

L-methionine salvage cycle I (bacteria and plants) (11/12 steps found)
L-methionine salvage cycle II (plants) (9/11 steps found)
S-methyl-5'-thioadenosine degradation I (2/2 steps found)
5'-deoxyadenosine degradation II (3/4 steps found)

KEGG Metabolic Maps

Methionine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.100

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (399 amino acids)

>EX28DRAFT_2359 5'-methylthioribose kinase (EC 2.7.1.100) (Enterobacter asburiae PDN3)
MSQYRTFTAQDAVEYARQFGGLDDPSSLVEAQEVGDGNLNLVFKIFDGAGVSRIVVKQAL
PYVRCVGESWPLTLDRARLEAQTLVEHYQHSPQHTVKIHHFDPELAVMVMEDLSSHRIWR
GELINNIYYPQAARQLGEYLAHALFHTSDFFLHPHAKKAQVAKFINPEMCEITEDLFFND
PYQIHERNSYPAELENDVAALRDDAQLKIAVASLKHRFFSHAEALLHGDIHSGSIFVADG
SLKAIDAEFGYFGPIGFDIGTAIGNLLLNFCGLPGHLGIRDAAAAREQRLTDIQELWNTF
AERFQALANEKTRDAALGAPGYASAFLKKVWHDAIGFCGTELIRRSVGLSHVADIDTIQD
EAMRHECLRHAITLGKALIVIADRIDSAEDLVARVRQYS