Protein Info for EX28DRAFT_2328 in Enterobacter asburiae PDN3

Annotation: penicillin-binding protein 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 633 transmembrane" amino acids 21 to 41 (21 residues), see Phobius details TIGR03423: penicillin-binding protein 2" amino acids 19 to 612 (594 residues), 794.5 bits, see alignment E=3.2e-243 PF03717: PBP_dimer" amino acids 64 to 239 (176 residues), 162.9 bits, see alignment E=1.1e-51 PF00905: Transpeptidase" amino acids 272 to 608 (337 residues), 268.4 bits, see alignment E=7.7e-84

Best Hits

Swiss-Prot: 93% identical to MRDA_ECOLI: Peptidoglycan D,D-transpeptidase MrdA (mrdA) from Escherichia coli (strain K12)

KEGG orthology group: K05515, penicillin-binding protein 2 (inferred from 98% identity to enc:ECL_03060)

MetaCyc: 93% identical to peptidoglycan DD-transpeptidase MrdA (Escherichia coli K-12 substr. MG1655)
Serine-type D-Ala-D-Ala carboxypeptidase. [EC: 3.4.16.4]

Predicted SEED Role

"Penicillin-binding protein 2 (PBP-2)" in subsystem Peptidoglycan Biosynthesis

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.16.4

Use Curated BLAST to search for 3.4.16.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (633 amino acids)

>EX28DRAFT_2328 penicillin-binding protein 2 (Enterobacter asburiae PDN3)
MKLQNSFRDYTAESALFVRRALVAFTGILLLTGVLIANLYNLQIVRFTDYQTRSNENRIK
LVPIAPSRGIIYDRNGTPLALNRTIYQIEMMPEKVDNVQDTLEALKSVVDLNDDDIAAFK
KERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKGYKRRYYPYGSALTHVIGYVS
KINDKDVDRLDKDGKLANYAATHDIGKLGIERYYEDVLHGQTGYEEVEVNNRGRVIRQLK
EVPPQAGHDVYLTLDLKLQQYIETLLAGSRAAVVVTDPRTGGILAMVSMPSYDPNLFVDG
ISSKDYSGLLNDPNTPLVNRATQGVYPPASTVKPYVAVSALSAGVINRNTSLFDPGWWQL
PGSDKRYRDWKKWGHGHLNVTKSLEESADTFFYQVAYDMGIDRLSEWMSKFGYGHYTGVD
LSEERSGNMPTREWKLKRFKKPWYQGDTIPVGIGQGYWTATPIQMNKAMMILINDGVVKV
PHLLQSTVEDGKKVPWNQPHEAPVGDIHSGFWEIAKDGMYGVANRPNGTAHKYFAGTPYK
VAAKSGTAQVFGLKANETYNAHRIAERLRDHKLMTAFAPYDNPQVAVAMILENGGAGPAV
GTIMRQILDHIMLGDNNTELPAENPAAAAAEDQ