Protein Info for EX28DRAFT_2322 in Enterobacter asburiae PDN3

Annotation: leucyl-tRNA synthetase (EC 6.1.1.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 909 transmembrane" amino acids 570 to 590 (21 residues), see Phobius details TIGR00396: leucine--tRNA ligase" amino acids 54 to 908 (855 residues), 1298.3 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 63 to 274 (212 residues), 109 bits, see alignment E=5.1e-35 amino acids 467 to 621 (155 residues), 32 bits, see alignment E=1.2e-11 amino acids 667 to 701 (35 residues), 27.5 bits, see alignment (E = 2.7e-10) PF09334: tRNA-synt_1g" amino acids 88 to 232 (145 residues), 81.8 bits, see alignment E=8.6e-27 amino acids 656 to 708 (53 residues), 21 bits, see alignment 2.6e-08 PF13603: tRNA-synt_1_2" amino acids 270 to 452 (183 residues), 267.1 bits, see alignment E=1.3e-83 PF08264: Anticodon_1" amino acids 749 to 871 (123 residues), 69.8 bits, see alignment E=5e-23

Best Hits

Swiss-Prot: 94% identical to SYL_CITK8: Leucine--tRNA ligase (leuS) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)

KEGG orthology group: K01869, leucyl-tRNA synthetase [EC: 6.1.1.4] (inferred from 93% identity to ecv:APECO1_14132)

MetaCyc: 94% identical to leucine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Leucine--tRNA ligase. [EC: 6.1.1.4]; 6.1.1.4 [EC: 6.1.1.4]; 3.1.1.- [EC: 6.1.1.4]

Predicted SEED Role

"Leucyl-tRNA synthetase (EC 6.1.1.4)" (EC 6.1.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (909 amino acids)

>EX28DRAFT_2322 leucyl-tRNA synthetase (EC 6.1.1.4) (Enterobacter asburiae PDN3)
MKHRPGKFLQSAFVRKAVLTRAFGLCYADLNYHPLATFVAVLKTGPLAAMQEQYRPEEIE
SKVQQHWDEKRTFEVTEDESKEKYYCLSMLPYPSGRLHMGHVRNYTIGDVIARYQRMLGK
NVLQPIGWDAFGLPAEGAAVKNNTAPAPWTYDNIAYMKNQLKMLGFGYDWSRELATCTPE
YYRWEQKFFTELYKKGLVYKKTSAVNWCPNDQTVLANEQVIDGCCWRCDTKVERKEIPQW
FIKITAYADELLNDLDNLDHWPDTVKTMQRNWIGRSEGVEITFNVENYDQTLTVYTTRPD
TFMGATYLAVAAGHPLAQKAAENNPELATFIDECRNTKVAEADMATMEKKGVDTGFKAIH
PLTGEAIPVWAANFVLMEYGTGAVMAVPGHDQRDYEFATKYGLTIKPVILAADGSEPDLS
EHALTEKGTLFNSGEFSGLSFEEGFNAIADKLASLGVGERKVNFRLRDWGVSRQRYWGAP
IPMVTLEDGSVMPTPEDQLPVILPEDVVMDGITSPIKADPEWAKTTVNGQPALRETDTFD
TFMESSWYYARYTCPQYQDGMLDSDAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDA
GMVNSDEPAKQLLCQGMVLADAFYYVGANGERNWVSPVDAIVERDEKGRIVKAKDAEGHE
LVYTGMSKMSKSKNNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLK
RVWKLVYEHTALGDAPALNAAALTEDQQALRRDVHKTIAKVTDDIGRRQTFNTAIAAIME
LMNKLAKAPQDGEQDRALMREALLAVVRMLNPFTPHVSFTLWQELKGEGDIDNAPWPQAD
ESAMVENTTLVVVQVNGKVRGKITVAVDATEEQVRERAGQEPLVAKYLEGVTVRKVIYVP
GKLLNLVVG