Protein Info for EX28DRAFT_2286 in Enterobacter asburiae PDN3

Annotation: putrescine-ornithine antiporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 437 transmembrane" amino acids 9 to 28 (20 residues), see Phobius details amino acids 38 to 58 (21 residues), see Phobius details amino acids 88 to 108 (21 residues), see Phobius details amino acids 120 to 139 (20 residues), see Phobius details amino acids 150 to 170 (21 residues), see Phobius details amino acids 183 to 203 (21 residues), see Phobius details amino acids 223 to 247 (25 residues), see Phobius details amino acids 274 to 298 (25 residues), see Phobius details amino acids 319 to 340 (22 residues), see Phobius details amino acids 349 to 372 (24 residues), see Phobius details amino acids 384 to 402 (19 residues), see Phobius details amino acids 408 to 426 (19 residues), see Phobius details TIGR00905: transporter, basic amino acid/polyamine antiporter (APA) family" amino acids 3 to 431 (429 residues), 500.8 bits, see alignment E=3.9e-154 TIGR04299: putrescine-ornithine antiporter" amino acids 3 to 431 (429 residues), 825.8 bits, see alignment E=9.6e-253 PF13520: AA_permease_2" amino acids 8 to 402 (395 residues), 164.5 bits, see alignment E=4.1e-52 PF00324: AA_permease" amino acids 13 to 401 (389 residues), 76.6 bits, see alignment E=1.7e-25

Best Hits

Swiss-Prot: 93% identical to POTE_ECOLI: Putrescine transporter PotE (potE) from Escherichia coli (strain K12)

KEGG orthology group: K03756, putrescine:ornithine antiporter (inferred from 100% identity to enc:ECL_03026)

MetaCyc: 93% identical to putrescine transporter PotE (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-69; TRANS-RXN0-211

Predicted SEED Role

"Putrescine/proton symporter, putrescine/ornithine antiporter PotE" in subsystem Polyamine Metabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (437 amino acids)

>EX28DRAFT_2286 putrescine-ornithine antiporter (Enterobacter asburiae PDN3)
MSKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIISWLVTAVGSMALAWAFAKC
GMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVAIAISAVGYGTELFGATLSPV
QIGLATIGVLWICTVANFGGARITGQLSSITVWGVIIPVVGLCIIGWFWFSPTLYANSWN
PHHVPFFTAVGSSIAMTLWAFLGLESACANAEVVENPEKNVPIAVLGGTLGAAVIYIVST
NVIAGIVPNMDLANSTAPFGLAFAQMFTPEVGKVIMGLMVMSCCGSLLGWQFTIAQVFKS
SADEGYFPKIFSRVTKADAPVQGMLAIVIFQSGLSLMTISPSLNSQFNVLVNLAVVTNII
PYILSMAALVIIQKVAKVDPRKARAANIVALIGAIYSFYALYSSGQEAMLYGAMVTFMGW
TLYGLVSPRFELKNKHS