Protein Info for EX28DRAFT_2219 in Enterobacter asburiae PDN3
Annotation: histidine ammonia-lyase (EC 4.3.1.3)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 92% identical to HUTH_ENT38: Histidine ammonia-lyase (hutH) from Enterobacter sp. (strain 638)
KEGG orthology group: K01745, histidine ammonia-lyase [EC: 4.3.1.3] (inferred from 97% identity to enc:ECL_02964)MetaCyc: 80% identical to histidase subunit (Pseudomonas putida)
Histidine ammonia-lyase. [EC: 4.3.1.3]
Predicted SEED Role
"Histidine ammonia-lyase (EC 4.3.1.3)" in subsystem Histidine Degradation (EC 4.3.1.3)
MetaCyc Pathways
- L-histidine degradation VI (7/8 steps found)
- L-histidine degradation I (4/4 steps found)
- L-histidine degradation III (4/6 steps found)
- L-histidine degradation II (3/5 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 4.3.1.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (506 amino acids)
>EX28DRAFT_2219 histidine ammonia-lyase (EC 4.3.1.3) (Enterobacter asburiae PDN3) MNALTLTPGSLTLKQLRNVWRKPVTLSLDATAHAAINDSVACVEAIVAEGRTAYGINTGF GLLAQTRIATHDLENLQRSLVLSHAAGVGQPLDDEIVRLMMVLKINSLARGFSGIRLSVI QALMALVNAEVYPWIPAKGSVGASGDLAPLAHMSLLLLGEGKARWQGEWLPAKEALKKAG LNPITLAAKEGLALLNGTQASTAFALRGLFEAEDLFASAVVCGALTTEAVLGSRRPFDAR IHEVRGQRGQIDAAAMYRHVLTDTSEIADSHHNCEKVQDPYSLRCQPQVMGACLTQLRQA AEVLLVEANAVSDNPLVFAQENEVVSGGNFHAEPVAMAADNIALAIAEVGALSERRIALM MDKHMSQLPPFLVRNGGVNSGFMIAQVTAAALASENKALSHPHSVDSLPTSANQEDHVSM APAAGRRLWEMASNTRGVLAVEWLAACQGVDLREGLKSSPLLEQARHALREHVSHYDDDR FFAPDIDKAMQLLEEGSLVGLLPSVL