Protein Info for EX28DRAFT_2215 in Enterobacter asburiae PDN3

Annotation: 8-amino-7-oxononanoate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00858: 8-amino-7-oxononanoate synthase
  • amino acids 20 to 381 (362 residues), 470.7 bits, E=1.3e-145
• PF00155: Aminotran_1_2
  • amino acids 41 to 380 (340 residues), 226.7 bits, E=5.2e-71
• PF00266: Aminotran_5
  • amino acids 80 to 210 (131 residues), 25.4 bits, E=7.1e-10

Best Hits

Swiss-Prot: 80% identical to BIOF_ENT38: 8-amino-7-oxononanoate synthase (bioF) from Enterobacter sp. (strain 638)

KEGG orthology group: K00652, 8-amino-7-oxononanoate synthase [EC: 2.3.1.47] (inferred from 87% identity to enc:ECL_02960)

MetaCyc: 74% identical to 8-amino-7-oxononanoate synthase (Escherichia coli K-12 substr. MG1655)
8-amino-7-oxononanoate synthase. [EC: 2.3.1.47]; 2.3.1.47 [EC: 2.3.1.47]

Predicted SEED Role

"8-amino-7-oxononanoate synthase (EC 2.3.1.47)" in subsystem Biotin biosynthesis (EC 2.3.1.47)

MetaCyc Pathways

• biotin biosynthesis I (15/15 steps found)
• 8-amino-7-oxononanoate biosynthesis I (11/11 steps found)
• 8-amino-7-oxononanoate biosynthesis IV (4/5 steps found)
• biotin biosynthesis II (4/6 steps found)
• 8-amino-7-oxononanoate biosynthesis II (1/2 steps found)
• 8-amino-7-oxononanoate biosynthesis III (1/2 steps found)

KEGG Metabolic Maps

• Biotin metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.47

Protein Sequence (385 amino acids)

>EX28DRAFT_2215 8-amino-7-oxononanoate synthase (Enterobacter asburiae PDN3)
MTWQARINTALDERRAAEAFRVRRVVENGAGRFLTREGRQFCNFSSNDYLGLSQHPAIVR
AWQQGAERFGVGSGGSGHVSGYTTAHQALEEALADWLGYPRALLFISGFAANQAVITALM
GKEDRIVADRLSHASLLEAANLSPAQLRRFAHNDAGQLAALLDKPCDGQQLAVTEGVFSM
DGDSAPLAALHEAAKRQNAWLLVDDAHGIGVMGDEGRGSAHQQNVKPELLIVTFGKGFGV
SGAAVLCSDSVADYLLQFARHLIYSTSMPPAQAVALSASLAVIRSAEGDERRQRLAEHIS
RFRQGLAALPFHSPDSQSAIQPVIVGENGRALALAQALRERGMWVTAIRPPTVPPGTARL
RLTLTAAHEARDIQTLLEVLHDAGE