Protein Info for EX28DRAFT_2193 in Enterobacter asburiae PDN3

Annotation: Rad3-related DNA helicases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 725 PF00270: DEAD" amino acids 31 to 95 (65 residues), 24.5 bits, see alignment E=4e-09 PF06733: DEAD_2" amino acids 195 to 264 (70 residues), 31.8 bits, see alignment E=2.3e-11 PF13307: Helicase_C_2" amino acids 531 to 688 (158 residues), 124.6 bits, see alignment E=9.1e-40

Best Hits

Swiss-Prot: 88% identical to DING_ECOL6: ATP-dependent DNA helicase DinG (dinG) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K03722, ATP-dependent DNA helicase DinG [EC: 3.6.4.12] (inferred from 96% identity to enc:ECL_02935)

MetaCyc: 88% identical to ATP-dependent DNA helicase DinG (Escherichia coli K-12 substr. MG1655)
RXN0-4261 [EC: 5.6.2.3]

Predicted SEED Role

"ATP-dependent helicase DinG/Rad3" in subsystem DNA repair, bacterial DinG and relatives

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12 or 5.6.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (725 amino acids)

>EX28DRAFT_2193 Rad3-related DNA helicases (Enterobacter asburiae PDN3)
MALTAALKAQIGAWYKALQQQIPDFIPRAPQRQMIADVAKTLAGDDGRHLAIEAPTGVGK
TLSYLIPGIAIAREEDKTLVVSTANVALQDQIFSKDLPLLRKIIPDLRFTAAFGRGRYVC
PRNLAALASSEPNQQDLLAFLDDELTPNNKAEQEQCAKLKAELDGYKWDGLRDHTSQAIS
DDLWRRLSTDKASCLNRNCHYYRECPFFVARREIQEAEVVVANHALVMAALESEAVLPEP
KNLLLVLDEGHHLPDVARDALEMSAEITAPWFRLQLDLFCKLVATCMEQFRPKTTPPLAV
PERLSDHCEEVYGLIASLNNILNLYLPATQEAEHRFAMGELPEEVMAICQHLAKHLEKLR
GLAEMFLNDLSEKTGSHDVVRLHRVLLQMNRALGMFEAQSKLWRLASMAQASGAPVTKWA
TRDVRDGQVHLFFHCVGIRVADQLEKLIWRSVPHVVVTSATLRSLNSFSRLQEMSGLKEK
AGDRFVALDSPFNHCEQGKLVIPRMKFEPLIDNEEQHIAEMAAYFREQVESKKYPGMLVL
FASGRAMQRFLEHVTDLRLLLLVQGDQPRYRLVETHRKRIDNGERSVLVGLQSFAEGLDL
KGDYLTQVHIHKIAFPPIDSPVVITEGEWLKSLNRYPFEVQSLPAASFNLIQQVGRLIRS
HGCWGEVVIYDKRLLTKNYGQRLLNALPIFPIEQPEVPEVKKRPATLTSGRKKSIRAKGR
GPTGK