Protein Info for EX28DRAFT_2166 in Enterobacter asburiae PDN3

Annotation: drug resistance transporter, EmrB/QacA subfamily

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 523 transmembrane" amino acids 16 to 40 (25 residues), see Phobius details amino acids 56 to 78 (23 residues), see Phobius details amino acids 84 to 106 (23 residues), see Phobius details amino acids 142 to 164 (23 residues), see Phobius details amino acids 170 to 192 (23 residues), see Phobius details amino acids 206 to 226 (21 residues), see Phobius details amino acids 238 to 260 (23 residues), see Phobius details amino acids 280 to 300 (21 residues), see Phobius details amino acids 309 to 331 (23 residues), see Phobius details amino acids 340 to 357 (18 residues), see Phobius details amino acids 376 to 395 (20 residues), see Phobius details amino acids 487 to 512 (26 residues), see Phobius details TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 18 to 504 (487 residues), 430.9 bits, see alignment E=3.1e-133 PF00083: Sugar_tr" amino acids 18 to 197 (180 residues), 54 bits, see alignment E=1.4e-18 PF07690: MFS_1" amino acids 23 to 418 (396 residues), 184.5 bits, see alignment E=2.8e-58

Best Hits

KEGG orthology group: K03446, MFS transporter, DHA2 family, multidrug resistance protein B (inferred from 98% identity to enc:ECL_02910)

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (523 amino acids)

>EX28DRAFT_2166 drug resistance transporter, EmrB/QacA subfamily (Enterobacter asburiae PDN3)
MTDHSHDSWKPASNPWAVAIVVTLAVFMEILDTTIVNVALPHVAGSLSSSYDESTWVLTS
YLVANGIVLPISAFLSRVFGRKQFFLICIVMFTVCSFLCGIATELWQIILFRVMQGFFGG
GLQPTQQSVLLDYFKPEDRGKAFGLSSIAIIVAPVLGPTLGGWITDNYSWRWVFFINIPV
GIVTVLAIYQLLEDPPWESKSKEKLTIDWTGIGLIALGLGCLQVMLDRGEDEDWFYSNFI
RTFGVLTLVGIIGAIYWLVYARKPVVDLHCMKDRNFAISSLLMAGMAMILYGSSVVIPQL
AQQDLGYTATWSGLVLSPGAVLIVLTIPLVLKLMPVVQTRWIIAFGFTCLAVSFFWSRTL
TPDIDFETLVLFRSAQSIGLGFLFVPLTTIAFISIPRRLNADAAALFTMFRNVAGSIGIS
LSTAAITERAQAHSAHLAYHASPLNEQFLQAIRESAQAIQNFTTQVGDPTGIATGRMYQT
MIEQSRFLAYIDVFTILSAVALLLIPFCLLLSPVKSEGSAGAH