Protein Info for EX28DRAFT_2123 in Enterobacter asburiae PDN3

Annotation: Major Facilitator Superfamily

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 410 signal peptide" amino acids 12 to 14 (3 residues), see Phobius details transmembrane" amino acids 15 to 31 (17 residues), see Phobius details amino acids 53 to 72 (20 residues), see Phobius details amino acids 84 to 103 (20 residues), see Phobius details amino acids 110 to 130 (21 residues), see Phobius details amino acids 142 to 165 (24 residues), see Phobius details amino acids 172 to 189 (18 residues), see Phobius details amino acids 221 to 240 (20 residues), see Phobius details amino acids 258 to 276 (19 residues), see Phobius details amino acids 287 to 308 (22 residues), see Phobius details amino acids 313 to 337 (25 residues), see Phobius details amino acids 348 to 367 (20 residues), see Phobius details amino acids 379 to 399 (21 residues), see Phobius details PF07690: MFS_1" amino acids 25 to 364 (340 residues), 105.5 bits, see alignment E=2.9e-34 PF00083: Sugar_tr" amino acids 58 to 195 (138 residues), 25.8 bits, see alignment E=5.1e-10

Best Hits

Swiss-Prot: 89% identical to MDFA_ECO57: Multidrug transporter MdfA (mdfA) from Escherichia coli O157:H7

KEGG orthology group: K08160, MFS transporter, DHA1 family, multidrug/chloramphenicol efflux transport protein (inferred from 97% identity to enc:ECL_02875)

MetaCyc: 89% identical to multidrug efflux pump MdfA / Na+:H+ antiporter / K+:H+ antiporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-101; TRANS-RXN-337; TRANS-RXN-338; TRANS-RXN-42; TRANS-RXN-44

Predicted SEED Role

"Multidrug translocase MdfA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (410 amino acids)

>EX28DRAFT_2123 Major Facilitator Superfamily (Enterobacter asburiae PDN3)
MLNRSSSGSRLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVVAQYNAGIEWVPTSMTA
YLAGGMFLQWLLGPLSDRIGRRPVMLTGVVWFIVTCLATLLAQNIEQFTLLRFLQGVSLC
FIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLVGAAWVHVAPWEGMFVLFALL
AAFAFFGLHRAMPETATRIGEKLSLKELGRDYKEVLKNGRFVAGALATGFVSLPLLAWIA
QSPVIIISGEKLSSYEYGLLQVPIFGALIIGNLVLARLTSRRTVRSLIIMGGWPIAAGLI
LAAVATVASSHAYLWMTAGLSLYAFGIGVANAGLVRLTLFASEMSKGTVSAAMGMLQMLI
FTVGIEVSKHAYAMGGNGLFSLFNLANGVLWVGLMVVFLKDKRVGNALQP