Protein Info for EX28DRAFT_2068 in Enterobacter asburiae PDN3

Annotation: Chitinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 908 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF08329: ChitinaseA_N" amino acids 22 to 153 (132 residues), 190.2 bits, see alignment E=3.5e-60 PF00704: Glyco_hydro_18" amino acids 158 to 546 (389 residues), 324 bits, see alignment E=4.5e-100 PF21027: Sde0182_C" amino acids 589 to 651 (63 residues), 32 bits, see alignment 4.7e-11 PF00801: PKD" amino acids 680 to 749 (70 residues), 27.6 bits, see alignment 6.4e-10 PF02839: CBM_5_12" amino acids 814 to 852 (39 residues), 32.7 bits, see alignment (E = 2e-11) amino acids 861 to 898 (38 residues), 30.5 bits, see alignment (E = 9e-11)

Best Hits

KEGG orthology group: K01183, chitinase [EC: 3.2.1.14] (inferred from 82% identity to cko:CKO_02217)

Predicted SEED Role

"Chitinase (EC 3.2.1.14)" in subsystem Chitin and N-acetylglucosamine utilization (EC 3.2.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.14

Use Curated BLAST to search for 3.2.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (908 amino acids)

>EX28DRAFT_2068 Chitinase (Enterobacter asburiae PDN3)
MKFLKPKYLALFVAAAASPVFAAVPGKATIASGNDKFAIVEVDQSATAYNSLVKVHDGAD
VKVQWDVWSGAAPTSAKVLLDGKTVWTGAGSMSGTATFKVTKGGRYQETVELCNASGCST
SASKLIIVADTDGSHLLPLNTTMQENNKTLSKHTDKVVGAYFPEWGVYDRKFPVDKIPAA
NLNHILYGFIPICGGDGINDSLKTIEGGNSFEALKRACAGRQDFQVAIHDPWAALQESRT
GLDSWDEPYKGNFGQLMAMKKANPGLKVLPSIGGWTLSDPFFKMHDKAIRDRFVASVKDF
LKTWKFFDGVDIDWEFPGGGGQNETLGNPQQDKETYTALMRELRAMMNELSAETGRTFEL
TSAIGSGSDKIEDVDYTTAQQYMDHIFLMSYDFYGGWSNTDLGHQAALRAPANKPDTNYT
TENGVNALLAQGVQPGKIVVGTAMYGRGWTGVHGYSNNNPFTGTATGMVKGTWEPGVVDY
RQIVNEYKGKAGWEYNYDATAEAPYLFNKTTGDLITYEDARSATAKGQYVLAKNLGGLFA
WSIDSDTGDILNAMNESLTGGGAAPVDPVVTNRAPVATSADQSVTGPATVTLDGSASTDP
DGDAITYKWTQISGPSVTLTNSTKAKATFNVAAVTSNQTLAFRLTVTDAKGLSSTADVQV
VNKAPKANQAPVVNQMEAVTLEAGQSYSLHAQANDPDGDALTYSWSVPANMQATGADTAN
LNITAPDVDTESTYTLTVVVSDGKSSVQTNVQVTVTPKAAETPSDEGNTPSDEGNTPSDE
GNTPSDEGNTPSDEGSATNSCDKPVDANASKYAAWSASKVYNAGNTVSFDNLVWKAKYWT
QGNQPGFGVDAWELVSQVKFNWRSDVVYNGGDTTTYNGYTYTAKWWTKGDKPGSSDVWVK
KGPAADCQ