Protein Info for EX28DRAFT_2062 in Enterobacter asburiae PDN3

Annotation: Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02776: TPP_enzyme_N
  • amino acids 4 to 115 (112 residues), 111.6 bits, E=3e-36
• PF00205: TPP_enzyme_M
  • amino acids 191 to 319 (129 residues), 117.2 bits, E=6.7e-38
• PF02775: TPP_enzyme_C
  • amino acids 379 to 525 (147 residues), 144.1 bits, E=4.6e-46

Best Hits

Swiss-Prot: 95% identical to POXB_ECOLI: Pyruvate dehydrogenase [ubiquinone] (poxB) from Escherichia coli (strain K12)

KEGG orthology group: K00156, pyruvate dehydrogenase (quinone) [EC: 1.2.5.1] (inferred from 95% identity to eco:b0871)

MetaCyc: 95% identical to pyruvate oxidase (Escherichia coli K-12 substr. MG1655)
RXN-11496 [EC: 1.2.5.1]; RXN0-2022 [EC: 1.2.5.1]

Predicted SEED Role

"Pyruvate oxidase [ubiquinone, cytochrome] (EC 1.2.2.2)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 1.2.2.2)

MetaCyc Pathways

• pyruvate to cytochrome bd oxidase electron transfer (2/2 steps found)
• pyruvate fermentation to acetoin (2/3 steps found)
• pyruvate to cytochrome bo oxidase electron transfer (1/2 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.2.2 or 1.2.5.1

Protein Sequence (572 amino acids)

>EX28DRAFT_2062 Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase] (Enterobacter asburiae PDN3)
MKQTVAAYIAKTLEQAGVKRIWGVTGDSLNGLSDSLNKMKTIEWMPTRHEEVAAFAAGAE
AQLTGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQEL
FRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVIPGDVALKAAPEGASTHWYHAP
QPVVTPAEEELKKLAQLLRYSSNIALMCGSGCAGAHKELLEFAGKLKSPIVHALRGKEHV
EYDNPYDVGMTGLIGFSSGFHTMMNADTLILLGTQFPYRPFYPADAKIIQIDINPASIGA
HSKVDMALVGDIKSTLSALLPLLEEKTDRKFLDKALSDYRDARKGLDDLAKPSDKAIHPQ
YLAQQISHFADDDAIFTCDVGTPTVWAARYLKMNGKRRLLGSFNHGSMANAMPQALGAKA
TAPERQVVAMCGDGGFSMLMGDFLSVVQMKLQLKIVVFNNSVLGFVAMEMKAGGYLTDGT
ELHDTNFARIAEACGITGIRVEKASEMDEALQRAFSIDGPVLVDVVVAKEELAIPPQIKL
EQAKGFSLYMLRAIISGRGDEVIELAKTNWLR