Protein Info for EX28DRAFT_2050 in Enterobacter asburiae PDN3

Annotation: ATP-dependent Clp protease ATP-binding subunit ClpA (EC 3.4.21.92)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 759 PF02861: Clp_N" amino acids 2 to 126 (125 residues), 106.4 bits, see alignment E=6.8e-34 TIGR02639: ATP-dependent Clp protease ATP-binding subunit ClpA" amino acids 3 to 735 (733 residues), 1096 bits, see alignment E=0 PF00158: Sigma54_activat" amino acids 190 to 291 (102 residues), 20.4 bits, see alignment E=2e-07 amino acids 490 to 613 (124 residues), 20.4 bits, see alignment E=2e-07 PF05621: TniB" amino acids 195 to 347 (153 residues), 31.1 bits, see alignment E=9e-11 PF00004: AAA" amino acids 212 to 344 (133 residues), 51.6 bits, see alignment E=7.5e-17 amino acids 493 to 608 (116 residues), 36.4 bits, see alignment E=3.7e-12 PF17871: AAA_lid_9" amino acids 351 to 451 (101 residues), 100 bits, see alignment E=3.8e-32 PF07724: AAA_2" amino acids 487 to 648 (162 residues), 198.9 bits, see alignment E=3.4e-62 PF07728: AAA_5" amino acids 492 to 610 (119 residues), 50.4 bits, see alignment E=1.3e-16 PF10431: ClpB_D2-small" amino acids 654 to 734 (81 residues), 91.9 bits, see alignment E=1.1e-29

Best Hits

Swiss-Prot: 96% identical to CLPA_ECO57: ATP-dependent Clp protease ATP-binding subunit ClpA (clpA) from Escherichia coli O157:H7

KEGG orthology group: K03694, ATP-dependent Clp protease ATP-binding subunit ClpA (inferred from 100% identity to enc:ECL_02767)

MetaCyc: 96% identical to ATP-dependent Clp protease ATP-binding subunit ClpA (Escherichia coli K-12 substr. MG1655)
Endopeptidase Clp. [EC: 3.4.21.92]

Predicted SEED Role

"ATP-dependent Clp protease ATP-binding subunit ClpA" in subsystem Proteolysis in bacteria, ATP-dependent

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.92

Use Curated BLAST to search for 3.4.21.92

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (759 amino acids)

>EX28DRAFT_2050 ATP-dependent Clp protease ATP-binding subunit ClpA (EC 3.4.21.92) (Enterobacter asburiae PDN3)
MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREALEACSVDLVALRQELEA
FIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRSEVTGANVLVAIFSEQESQA
AYLLRKHEVSRLDVVNFISHGTRKDEPNQASDSSNQVNNNEEQAGGEDRMENFTTNLNQL
ARVGGIDPLIGRDKELERAIQVLCRRRKNNPLLVGESGVGKTAIAEGLAWRIVQGDVPEV
IADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQDTNSILFIDEIHTIIGAGAASGG
QVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKDRALARRFQKIDVTEPSVEETVQIIN
GLKPKYEAHHDVRYTAKAVRAAVELAVKYINDRHLPDKAIDVIDEAGARARLMPASKRKK
TVNVADIESVVARIARIPEKSVSQSDRDTLRTLGNRLKMLVFGQDKAIEALTEAIKMARA
GLGHDHKPVGSFLFAGPTGVGKTEVTVQLSKALGIELLRFDMSEYMERHTVSRLIGAPPG
YVGFDQGGLLTDAVIKHPHAVLLLDEIEKAHPDVFNILLQVMDNGTLTDNNGRKADFRNV
VLVMTTNAGVRETERKSIGLIHQDNSTDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIH
QVVDKFIVELQVQLDQKGVSLEVSQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANE
LLFGSLVDGGQVTVALDQAKNELTYDFQSAAKHKPEAAH