Protein Info for EX28DRAFT_2038 in Enterobacter asburiae PDN3

Annotation: anaerobic dimethyl sulfoxide reductase, A subunit, DmsA/YnfE family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 814 signal peptide" amino acids 1 to 46 (46 residues), see Phobius details TIGR02166: anaerobic dimethyl sulfoxide reductase, A subunit, DmsA/YnfE family" amino acids 14 to 814 (801 residues), 1394.9 bits, see alignment E=0 PF04879: Molybdop_Fe4S4" amino acids 56 to 116 (61 residues), 60.9 bits, see alignment 1.4e-20 PF00384: Molybdopterin" amino acids 119 to 576 (458 residues), 274.2 bits, see alignment E=2.4e-85 PF01568: Molydop_binding" amino acids 695 to 806 (112 residues), 95.8 bits, see alignment E=2.4e-31

Best Hits

Swiss-Prot: 88% identical to DMSA_ECOLI: Dimethyl sulfoxide reductase DmsA (dmsA) from Escherichia coli (strain K12)

KEGG orthology group: K07306, anaerobic dimethyl sulfoxide reductase subunit A [EC: 1.8.99.-] (inferred from 97% identity to enc:ECL_02756)

MetaCyc: 67% identical to putative selenate reductase YnfE (Escherichia coli K-12 substr. MG1655)
Selenate reductase. [EC: 1.97.1.9]

Predicted SEED Role

"Anaerobic dimethyl sulfoxide reductase chain A (EC 1.8.5.3)" (EC 1.8.5.3)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.8.5.3, 1.97.1.9

Use Curated BLAST to search for 1.8.5.3 or 1.8.99.- or 1.97.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (814 amino acids)

>EX28DRAFT_2038 anaerobic dimethyl sulfoxide reductase, A subunit, DmsA/YnfE family (Enterobacter asburiae PDN3)
MKINAPEALMAAEVTRRGLMKTTAIGGLALASSAFTLPFTRLASAADALSPASAPEKVVW
SACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYEGLHQVRACLRGRSMRRRVYNPDRL
KYPMKRVGKRGEGKFERISWDEAYDIIATNMQRLIKEYGNESIYLNYGTGTLGGTLTRSW
PPGKTLVARLMNCCGGYLNHYGDYSSAQIAAGLNYTYGGWADGNSPSDIENSKLVVLFGN
NPGETRMSGGGVTYYLEQARAKSNARMIIIDPRYTDTGAGREDEWIPIRPGTDAALVSAL
AWVMITENLVDQPFLDKYCVGYDEKTLPASAPANGHYKAYILGQGSDGVAKTPEWASTIT
GIPVERIVQLAREIGSAKPAYISQGWGPQRHANGEIATRAISMLSILTGNVGIHGGNTGA
REGSYEVPFERMPMLDNPVQTSISMFMWTDAIERGPEMTALRDGVRGKDKLDVPIKMIWN
YAGNCLINQHSEINRTHEILQDDKKCEMIVVIDCHMTSSAKYADILLPDCTASEQMDFAL
DASCGNMSYVIFTDQAIKPRFECKTIYEMTSELAKRLGVEQQFTEGRTQEGWMRHLHELS
RKTVPDLPDFDTFRKQGMYKQRDPEGHHVAYKAFREDPQANPLTTPSGKIEIYSEELAKI
ASTWELPEGDVIDPLPIYTPGFENYNDPLTAKFPLQLTGFHYKARVHSTYGNVDVLKAAC
RQEMWINPMDAKARGISNGDRVRIFNGRGEVHIEAKVTPRMMPGVVALGEGAWYSPDANR
IDQAGSINVLTTQRPSPLAKGNPSHTNLVQVEKV