Protein Info for EX28DRAFT_2028 in Enterobacter asburiae PDN3

Annotation: phosphoserine aminotransferase apoenzyme (EC 2.6.1.52)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01364: phosphoserine transaminase
  • amino acids 1 to 349 (349 residues), 490.3 bits, E=1.5e-151
• PF00266: Aminotran_5
  • amino acids 2 to 338 (337 residues), 213.8 bits, E=1.9e-67

Best Hits

Swiss-Prot: 93% identical to SERC_ENT38: Phosphoserine aminotransferase (serC) from Enterobacter sp. (strain 638)

KEGG orthology group: K00831, phosphoserine aminotransferase [EC: 2.6.1.52] (inferred from 98% identity to enc:ECL_02746)

MetaCyc: 91% identical to phosphoserine/phosphohydroxythreonine aminotransferase (Escherichia coli K-12 substr. MG1655)
Phosphoserine transaminase. [EC: 2.6.1.52]; 2.6.1.52 [EC: 2.6.1.52]; Succinyldiaminopimelate transaminase. [EC: 2.6.1.52, 2.6.1.17]

Predicted SEED Role

"Phosphoserine aminotransferase (EC 2.6.1.52)" in subsystem Glycine and Serine Utilization or Pyridoxin (Vitamin B6) Biosynthesis or Serine Biosynthesis (EC 2.6.1.52)

MetaCyc Pathways

• aspartate superpathway (24/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (18/18 steps found)
• superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (12/12 steps found)
• L-lysine biosynthesis I (9/9 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• pyridoxal 5'-phosphate biosynthesis I (7/7 steps found)
• superpathway of L-serine and glycine biosynthesis I (4/4 steps found)
• L-serine biosynthesis I (3/3 steps found)
• L-cysteine biosynthesis IX (Trichomonas vaginalis) (2/3 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism
• Lysine biosynthesis
• Vitamin B6 metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.17

Use Curated BLAST to search for 2.6.1.17 or 2.6.1.52

Protein Sequence (350 amino acids)

>EX28DRAFT_2028 phosphoserine aminotransferase apoenzyme (EC 2.6.1.52) (Enterobacter asburiae PDN3)
MLPADVLKQAQQELCDWNGLGTSVMEISHRGKEFIQVAEEAEKDFRDLLNIPSNYKVLFC
HGGGRGQFAGVPLNILGDKTTADYVDAGYWAASAVKEAHKYCTPNVIDAKVTVDGLRAVK
PMSEWQLSDNAAYLHYCPNETIDGIAIDETPNFGSDVVVAADFSSTILSAPIDVSRYGVI
YAGAQKNIGPAGLTIVVVREDLLGKAHKSCPSILDYTVLNDNDSMFNTPPTFAWYLSGLV
FKWLKQNGGVAQMDMVNQQKAELLYGVIDKSDFYRNDVAKSNRSRMNVPFQLADSSLDKV
FLEESFAAGLHALKGHRVVGGMRASIYNAMPLEGVKALTDFMIDFERRHG