Protein Info for EX28DRAFT_2022 in Enterobacter asburiae PDN3

Annotation: lipid A export permease/ATP-binding protein MsbA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 583 transmembrane" amino acids 21 to 43 (23 residues), see Phobius details amino acids 65 to 93 (29 residues), see Phobius details amino acids 142 to 162 (21 residues), see Phobius details amino acids 168 to 186 (19 residues), see Phobius details amino acids 246 to 270 (25 residues), see Phobius details amino acids 276 to 295 (20 residues), see Phobius details TIGR02203: lipid A export permease/ATP-binding protein MsbA" amino acids 12 to 581 (570 residues), 897.1 bits, see alignment E=2.3e-274 PF00664: ABC_membrane" amino acids 27 to 298 (272 residues), 212.8 bits, see alignment E=1.2e-66 PF00005: ABC_tran" amino acids 359 to 509 (151 residues), 120.4 bits, see alignment E=1.4e-38

Best Hits

Swiss-Prot: 96% identical to MSBA_ECOL5: Lipid A export ATP-binding/permease protein MsbA (msbA) from Escherichia coli O6:K15:H31 (strain 536 / UPEC)

KEGG orthology group: K11085, ATP-binding cassette, subfamily B, bacterial MsbA [EC: 3.6.3.-] (inferred from 97% identity to ent:Ent638_1433)

MetaCyc: 95% identical to ATP-binding lipopolysaccharide transport protein (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-236 [EC: 7.5.2.6]; 7.5.2.6 [EC: 7.5.2.6]

Predicted SEED Role

"Lipid A export ATP-binding/permease protein MsbA" in subsystem ZZ gjo need homes

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.-

Use Curated BLAST to search for 3.6.3.- or 7.5.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (583 amino acids)

>EX28DRAFT_2022 lipid A export permease/ATP-binding protein MsbA (Enterobacter asburiae PDN3)
MHNDKDLSTWQTFRRLWPMIAPFKTGLIVAGVALILNAASDTFMLSLLKPLLDDGFGKTD
RSVLLWMPLVVIGLMILRGITSYISSYCISWVSGKVVMTMRRRLFSHMMGMPVSFFDKQS
TGTLLSRITYDSEQVASSSSSALITVVREGASIIGLFAMMFYYSWQLSIILIVLAPIVSI
AIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVLIFGGQDVETKRFDKVSNKMRLQ
GMKMVSASSISDPIIQLIASLALAFVLYAASFPSVMETLTAGTITVVFSSMIALMRPLKS
LTNVNAQFQRGMAACQTLFSILDSEQEKDEGKRVIERANGDVEFRNVTFTYPGREVPALR
NINLSIPAGKTVALVGRSGSGKSTIASLMTRFYDINEGEILLDGHDLREYTLQSLRNQVA
LVSQNVHLFNDTVANNIAYARTDEYSREQIENAARMAYAMDFINKMDNGLDTIIGENGVL
LSGGQRQRIAIARALLRDSPILILDEATSALDTESERAIQSALDELQKNRTSLVIAHRLS
TIEQADEIVVVEDGVIVERGSHADLLEHRGVYAQLHKMQFGQQ