Protein Info for EX28DRAFT_2000 in Enterobacter asburiae PDN3

Annotation: ABC transporter, substrate-binding protein, aliphatic sulfonates family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 320 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF13379: NMT1_2" amino acids 27 to 254 (228 residues), 40.5 bits, see alignment E=5.9e-14 PF04069: OpuAC" amino acids 29 to 267 (239 residues), 38 bits, see alignment E=2.9e-13 TIGR01728: ABC transporter, substrate-binding protein, aliphatic sulfonates family" amino acids 30 to 309 (280 residues), 272.5 bits, see alignment E=1.9e-85 PF12974: Phosphonate-bd" amino acids 68 to 200 (133 residues), 39.3 bits, see alignment E=9.8e-14 PF09084: NMT1" amino acids 69 to 183 (115 residues), 47.4 bits, see alignment E=4.5e-16

Best Hits

Swiss-Prot: 84% identical to SSUA_ECOLI: Putative aliphatic sulfonates-binding protein (ssuA) from Escherichia coli (strain K12)

KEGG orthology group: K02051, sulfonate/nitrate/taurine transport system substrate-binding protein (inferred from 92% identity to enc:ECL_02703)

MetaCyc: 84% identical to aliphatic sulfonate ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-56-RXN [EC: 7.6.2.14]

Predicted SEED Role

"Alkanesulfonates-binding protein" in subsystem Alkanesulfonate assimilation or Alkanesulfonates Utilization or Putative sulfate assimilation cluster

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (320 amino acids)

>EX28DRAFT_2000 ABC transporter, substrate-binding protein, aliphatic sulfonates family (Enterobacter asburiae PDN3)
MFKTLTRIGLAGLMALTSLAHAAETAPESLRIGYQKGSVSMVLAKSHQLLEKRFPDTKFS
WVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVGVEPPKPKAEVILVPENS
DIKSVADLKGHKVAFQKGSSSHNLLLRALQEAGLKFTDIQPVYLTPADARAAFQQKNVDA
WAIWDPYYSAALLQGGVRVLKDGTTLKQTGSFYLAARPYAEKNGAFIQGVLDTFTQADAL
TQTQRQESIALLAKTMGLPEPVIASYLNHRPPTTITPVDAHVAALQQQTADLFYQNHLVP
KQVTIRERIWQPAGKEGAKS