Protein Info for EX28DRAFT_1917 in Enterobacter asburiae PDN3

Annotation: pyrimidine utilization protein D

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 266 transmembrane" amino acids 12 to 30 (19 residues), see Phobius details TIGR03611: pyrimidine utilization protein D" amino acids 1 to 257 (257 residues), 373.6 bits, see alignment E=2.4e-116 PF00561: Abhydrolase_1" amino acids 14 to 242 (229 residues), 79.5 bits, see alignment E=4.8e-26 PF12146: Hydrolase_4" amino acids 15 to 232 (218 residues), 53.2 bits, see alignment E=4e-18 PF12697: Abhydrolase_6" amino acids 16 to 247 (232 residues), 73.1 bits, see alignment E=8.3e-24

Best Hits

Swiss-Prot: 89% identical to RUTD_ENTCC: Putative aminoacrylate hydrolase RutD (rutD) from Enterobacter cloacae subsp. cloacae (strain ATCC 13047 / DSM 30054 / NBRC 13535 / NCDC 279-56)

KEGG orthology group: K09023, protein RutD (inferred from 89% identity to enc:ECL_02625)

Predicted SEED Role

"Possible hydrolase or acyltransferase RutD in novel pyrimidine catabolism pathway" in subsystem Pyrimidine utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (266 amino acids)

>EX28DRAFT_1917 pyrimidine utilization protein D (Enterobacter asburiae PDN3)
MKLSVSPPPYEGAPVVVFISGLGGSGSYWLPQLAALEPEYQVVCYDQRGTGNNPDTLPEG
YSLAQMADELAQALADAGIARYCVVGHALGALVGLQLALDRPDALTALVCVNGWLTLNAH
TRRCFQIRERLLHYGGAQAWVEAQPLFLYPADWMAARAPRLEAEEALALAHFQGKSNLLH
RLSALKKADFSRHAARIRCPVQIICSADDMLVPSVCSSELQAAIPHSRSAVMRQGGHACN
VTEPDTFNTLLLNGLASLLHSPEPAL