Protein Info for EX28DRAFT_1850 in Enterobacter asburiae PDN3

Annotation: flagellar hook-associated protein FlgK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 546 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details TIGR02492: flagellar hook-associated protein FlgK" amino acids 3 to 545 (543 residues), 335.2 bits, see alignment E=4.1e-104 PF00460: Flg_bb_rod" amino acids 5 to 35 (31 residues), 29 bits, see alignment (E = 1.2e-10) PF21158: flgK_1st_1" amino acids 336 to 415 (80 residues), 105.2 bits, see alignment E=2.2e-34 PF06429: Flg_bbr_C" amino acids 492 to 545 (54 residues), 26.9 bits, see alignment 6.2e-10

Best Hits

Swiss-Prot: 73% identical to FLGK_ECOLI: Flagellar hook-associated protein 1 (flgK) from Escherichia coli (strain K12)

KEGG orthology group: K02396, flagellar hook-associated protein 1 FlgK (inferred from 88% identity to enc:ECL_02556)

Predicted SEED Role

"Flagellar hook-associated protein FlgK" in subsystem Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (546 amino acids)

>EX28DRAFT_1850 flagellar hook-associated protein FlgK (Enterobacter asburiae PDN3)
MSSLINSAMSGLSAAQSALNTVSNNISSYNVAGYTRQTTVLGASNSTLTGGGWVGNGVYV
SGVQREYDAFITNQLRAAQTQSSGLTTRYQQMSKIDDVLSDTTNSLSTTLQDFFKSLQTL
VSNAEDPAARQTVLGKASGLVNQFKTNDQYLRDQDAQVNTAISSSVSQINNYAAQIANLN
DQISRLTGVGAGASPNDLLDQRDQLVTELNKIVGVEVSVQDSGTFNISLGNGYSLVQGSK
ASQLAAVKSSADPARTTIAYVDDVAGNIEIPEKLITTGSLGGLLTFRAEDLDKARNSLNQ
MALAFADAMNTQHEAGFDANGDAGGKLFNFGSPAVLSNSKNGGSAVVTASVTDSKQVQAT
DYKLQFNGTDWTVTRTSDKTSFTMSPDASGNLSFDGLNVNVSGTANTKDSFTVKPVSNVI
MNMDLAISDESKLAMASVQNGGESDNRNGQKLLDLQNGKVVGGNKTFNDAYASLVSTVGS
STASLKVSSQTKANVETQLIKQQQTISGVNLDEEYGNLQRYQQYYLANAQVLQTASTLFD
AIINIR