Protein Info for EX28DRAFT_1846 in Enterobacter asburiae PDN3

Annotation: ribonuclease, Rne/Rng family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1042 transmembrane" amino acids 113 to 130 (18 residues), see Phobius details TIGR00757: ribonuclease, Rne/Rng family" amino acids 13 to 425 (413 residues), 568.7 bits, see alignment E=3.7e-175 PF00575: S1" amino acids 36 to 117 (82 residues), 47.5 bits, see alignment 3.9e-16 PF10150: RNase_E_G" amino acids 121 to 391 (271 residues), 330.3 bits, see alignment E=1.9e-102 PF20833: RNase_E_G_Thio" amino acids 403 to 488 (86 residues), 107.9 bits, see alignment E=4.5e-35 PF12111: PNPase_C" amino acids 1003 to 1039 (37 residues), 68.4 bits, see alignment (E = 5.2e-23)

Best Hits

Swiss-Prot: 70% identical to RNE_ECOLI: Ribonuclease E (rne) from Escherichia coli (strain K12)

KEGG orthology group: K08300, ribonuclease E [EC: 3.1.26.12] (inferred from 76% identity to cko:CKO_01975)

MetaCyc: 70% identical to ribonuclease E (Escherichia coli K-12 substr. MG1655)
Ribonuclease E. [EC: 3.1.26.12]; 3.1.26.12 [EC: 3.1.26.12]; 3.1.26.12 [EC: 3.1.26.12]; 3.1.26.12 [EC: 3.1.26.12]; 3.1.26.12 [EC: 3.1.26.12]

Predicted SEED Role

"Ribonuclease E (EC 3.1.26.12)" in subsystem RNA processing and degradation, bacterial or Ribosome biogenesis bacterial (EC 3.1.26.12)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.26.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1042 amino acids)

>EX28DRAFT_1846 ribonuclease, Rne/Rng family (Enterobacter asburiae PDN3)
MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKGKITRIEPSLEAAFVDY
GAERHGFLPLKEIAREYFPANYNAHGRPNIKDVLREGQEVIVQIDKEERGNKGAALTTFI
SLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLELPDGMGLIVRTAGVGKSAEAL
QWDLSFRLKHWEAIQKAAESRPAPFLIHQESNVIVRAFRDYLRQDIGEILIDNPKVLELA
RQHIAALGRPDFTSKIKLYTGEIPLFSHYQIESQIESAFQREVRLPSGGSIVIDTTEALT
AIDINSARATRGGDIEETAYNTNLEAADEIARQLRLRDLGGLIVIDFIDMTPVRHQRAVE
NRLREAVRQDRARIQISHISRFGLLEMSRQRLSPSLGESSHHVCPRCSGTGTVRDNESLS
LSILRLIEEEALKENTKEVHAIVPVPVASYLLNEKRAAVSAIEARQGGVRCIIVPNDQMQ
TPHYHVLRVRKGEETTTLSYLLPKLHEEEMALPSDEEPAERKLPEQPALATFIMPEAPPE
AALEKPAAKPEAQKPEAAAAKAQPAQPGLLSRFFGALKKMFAEEEVQPEQPKEEPKAAKP
ERQQDRRKRQNNRRDRNDRSDRNDRNDRSERRDNRDNRSENGEGREQREDNRRNRREKQQ
PNVEDREIRQQAGDESEKSKQRDDQQPRRERSRRRNDEKRQAQQEVKNLNREEPVEQQDG
EQEERTQVMPRRKQRQLSQKVRFASEATEESGAIAVETSEIATGTQLAKVDLPAVVESNV
EQDDNGENRDNAGMPRRSRRSPRHLRVSGQRRRRYRDERYPTQSPMPLTIACASPEMASG
KVWIRYPVARPEQVVEEQPVTEEVIAPAAAVEAVATEAATVVEPQVVEAEAPHAVEVETT
HPEVIAAPVDAAPQLIAEEDAVVAEEVAEEAEPVAAVEDTAETLVETTAEEVVQDVEIQV
EPVVEEAKAPEVKPEPVVSAPAAAHVATAPMTRAPAPDYVPEAPRHSDWVRPAFNFDGKG
AAGGHSATHQATAPATRPQSVE