Protein Info for EX28DRAFT_1816 in Enterobacter asburiae PDN3

Annotation: transcription-repair coupling factor (mfd)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1148 PF17757: UvrB_inter" amino acids 129 to 217 (89 residues), 92.2 bits, see alignment 7.3e-30 TIGR00580: transcription-repair coupling factor" amino acids 147 to 1079 (933 residues), 1216.4 bits, see alignment E=0 PF21132: MFD_D3" amino acids 377 to 449 (73 residues), 93.4 bits, see alignment 2.6e-30 PF02559: CarD_TRCF_RID" amino acids 477 to 532 (56 residues), 67.4 bits, see alignment 3.8e-22 PF04851: ResIII" amino acids 600 to 758 (159 residues), 49.4 bits, see alignment E=2.1e-16 PF00270: DEAD" amino acids 602 to 764 (163 residues), 97.3 bits, see alignment E=3.5e-31 PF00271: Helicase_C" amino acids 802 to 906 (105 residues), 69.6 bits, see alignment E=1e-22 PF03461: TRCF" amino acids 1006 to 1098 (93 residues), 103 bits, see alignment E=3.8e-33

Best Hits

Swiss-Prot: 91% identical to MFD_ECOLI: Transcription-repair-coupling factor (mfd) from Escherichia coli (strain K12)

KEGG orthology group: K03723, transcription-repair coupling factor (superfamily II helicase) [EC: 3.6.4.-] (inferred from 64% identity to asu:Asuc_0557)

Predicted SEED Role

"Transcription-repair coupling factor" in subsystem DNA-replication or Transcription factors bacterial

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.-

Use Curated BLAST to search for 3.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1148 amino acids)

>EX28DRAFT_1816 transcription-repair coupling factor (mfd) (Enterobacter asburiae PDN3)
MPEHYRYSLPVKAGDQRQLGELTGAACATLVAEIAERHPGPVILVAPDMQNALRLHDEIR
QFTDSLVFSLADWETLPYDSFSPHQEIISSRLSTLYQLPTMQRGVLIVPVNTLMQRVCPH
SYLHGHALVMKKGQRLSRDALRAQLDGAGYRHVDQVMEHGEYATRGALLDLYPMGSDQPY
RLDFFDDEIDSLRVFDADTQRTLEEVESINLLPAHEFPTDKTAIELFRSQWRDKFEVKRD
AEHIYQQVSKGTLPAGIEYWQPLFFNEPLPALFSYFPANTLIVNTGDIDASASRFESETR
ARFENRGVDPMRPLLPPEALWLRTDELNAELKRWPRMQLKTDSLADKAANTNLAFRTLPD
LAVQAQQKSPLDNLRKFLESFTGPVVFSVESEGRREALGELLGRIKVAPKRILRLSEATG
NGRYLMIGAAEHGFIDTLNNLALICESDLLGERVARRRQDSRRTINPDTLIRNLAELHPG
QPIVHLEHGVGRYQGMTTLEAGGIKGEYLMLTYANDAKLYVPVSSLHLISRYAGGAEENA
PLHKLGGDAWARARQKAAEKVRDVAAELLDIYAQRAAKQGFAFKHDKEQYQLFCDSFPFE
TTPDQAQAINAVLSDMCQPLAMDRLVCGDVGFGKTEVAMRAAFLAVENNKQVAVLVPTTL
LAQQHFDNFRDRFANWPVRIEMLSRFRSTKEQTQILEQASEGKIDILIGTHKLLQSDVKW
KDLGLLIVDEEHRFGVRHKERIKAMRADVDILTLTATPIPRTLNMAMSGMRDLSIIATPP
ARRLAVKTFVREYDNLVVREAILREVLRGGQVYYLYNDVENIQKAADRLAELVPEARIAI
GHGQMRERELERVMNDFHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLR
GRVGRSHHQAYAWLLTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLG
EDQSGSMETIGFSLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDV
NTRLSFYKRIASAKKENELEEIKVELIDRFGLLPDPARNLLDIARLRQQAQKLGIRKLEG
NEKGGVIEFAEKNHVNPMWLIGLLQKQPQHFRLDGPTRLKFTQELTERKTRMDWVRNFMR
QLEENAIA