Protein Info for EX28DRAFT_1804 in Enterobacter asburiae PDN3

Annotation: spermidine/putrescine ABC transporter ATP-binding subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 361 PF00005: ABC_tran" amino acids 17 to 158 (142 residues), 129.9 bits, see alignment E=1.7e-41 TIGR01187: polyamine ABC transporter, ATP-binding protein" amino acids 31 to 355 (325 residues), 542.6 bits, see alignment E=1.5e-167 PF08402: TOBE_2" amino acids 275 to 355 (81 residues), 29.7 bits, see alignment E=8.3e-11

Best Hits

Swiss-Prot: 89% identical to POTA_SHIDS: Spermidine/putrescine import ATP-binding protein PotA (potA) from Shigella dysenteriae serotype 1 (strain Sd197)

KEGG orthology group: K11072, spermidine/putrescine transport system ATP-binding protein [EC: 3.6.3.31] (inferred from 96% identity to enc:ECL_02511)

MetaCyc: 89% identical to spermidine preferential ABC transporter ATP binding subunit (Escherichia coli K-12 substr. MG1655)
ABC-25-RXN [EC: 7.6.2.11, 7.6.2.16]; 7.6.2.11 [EC: 7.6.2.11, 7.6.2.16]

Predicted SEED Role

"Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1)" in subsystem Polyamine Metabolism (TC 3.A.1.11.1)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.31

Use Curated BLAST to search for 3.6.3.31 or 7.6.2.11 or 7.6.2.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (361 amino acids)

>EX28DRAFT_1804 spermidine/putrescine ABC transporter ATP-binding subunit (Enterobacter asburiae PDN3)
MQLERIRKSFDGKDVISDLTLTINDGEFLTLLGPSGCGKTTVLRLIAGLESVDNGHIHLE
NQDITQVPAEDRHVNTVFQSYALFPHMTVFENVAFGLRMQKTPAKEIEPRVTDALRMVQL
ETFAQRKPHQLSGGQQQRVAIARAVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKL
GITFVFVTHDQEEALTMSDRIVVMRDGKIEQDGTPREIYEEPKNLFVASFIGEINIFDAT
VIERLDDQRVRASVEGRECNITVTFPVEKGQKLNVLLRPEDLRVDEIHGNTEAEGLIGYI
RERNYKGMTLESVVELENGKMVMVSEFFNEDDPDFDHSLDQKMVINWVESWEVVLADEEH
K