Protein Info for EX28DRAFT_1797 in Enterobacter asburiae PDN3

Annotation: tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 370 PF02540: NAD_synthase" amino acids 4 to 76 (73 residues), 32.3 bits, see alignment E=2e-11 PF02568: ThiI" amino acids 5 to 76 (72 residues), 27.4 bits, see alignment E=8.4e-10 PF00733: Asn_synthase" amino acids 7 to 183 (177 residues), 22.2 bits, see alignment E=3.6e-08 TIGR00420: tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase" amino acids 7 to 361 (355 residues), 528.8 bits, see alignment E=2.7e-163 PF03054: tRNA_Me_trans" amino acids 7 to 203 (197 residues), 283.7 bits, see alignment E=2.9e-88 PF20259: tRNA_Me_trans_M" amino acids 208 to 276 (69 residues), 83.5 bits, see alignment E=2.2e-27 PF20258: tRNA_Me_trans_C" amino acids 287 to 361 (75 residues), 93.5 bits, see alignment E=2.8e-30

Best Hits

Swiss-Prot: 96% identical to MNMA_KLEP3: tRNA-specific 2-thiouridylase MnmA (mnmA) from Klebsiella pneumoniae (strain 342)

KEGG orthology group: K00566, tRNA-specific 2-thiouridylase [EC: 2.8.1.-] (inferred from 98% identity to enc:ECL_02501)

MetaCyc: 93% identical to tRNA-specific 2-thiouridylase (Escherichia coli K-12 substr. MG1655)
RXN0-2023 [EC: 2.8.1.13]

Predicted SEED Role

"tRNA-specific 2-thiouridylase MnmA"

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.-

Use Curated BLAST to search for 2.8.1.- or 2.8.1.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (370 amino acids)

>EX28DRAFT_1797 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (Enterobacter asburiae PDN3)
MSDNSQKKVIVGMSGGVDSSVSAYLLQQQGYKVEGLFMKNWEEDDGEEYCTAAADLADAQ
AVCDKLGIELHTVNFAAEYWDNVFELFLEEYKAGRTPNPDILCNKEIKFKAFLEFAAEDL
GADYIATGHYVRRADVNGKSQLLRGLDGNKDQSYFLYTLSHEQIAQSLFPVGELEKPQVR
KIAEELDLITAKKKDSTGICFIGERKFRDFLGRYLPAQPGKIVTVDGEEIGQHQGLMYHT
LGQRKGLGIGGTKEGSEDPWYVVDKDVENNILVVAQGHDHPRLLSVGLIAQQLHWVDREP
LKGTLRCTVKTRYRQTDIPCTITALDDDRIDVRFDEPVAAVTPGQSAVFYSGEICLGGGI
IEQRLPLPAV