Protein Info for EX28DRAFT_1752 in Enterobacter asburiae PDN3

Annotation: transaminase, acetylornithine/succinylornithine family/succinylornithine transaminase family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03246: succinylornithine transaminase family
  • amino acids 5 to 400 (396 residues), 730.4 bits, E=5.1e-224
• TIGR00707: transaminase, acetylornithine/succinylornithine family
  • amino acids 13 to 397 (385 residues), 538.2 bits, E=9.3e-166
• PF00202: Aminotran_3
  • amino acids 22 to 395 (374 residues), 430.7 bits, E=4.9e-133
• PF00155: Aminotran_1_2
  • amino acids 88 to 344 (257 residues), 21.5 bits, E=1.2e-08

Best Hits

Swiss-Prot: 89% identical to ASTC_CITK8: Succinylornithine transaminase (astC) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)

KEGG orthology group: K00840, succinylornithine aminotransferase [EC: 2.6.1.81] (inferred from 97% identity to enc:ECL_02449)

MetaCyc: 83% identical to succinylornithine transaminase (Escherichia coli K-12 substr. MG1655)
Succinylornithine transaminase. [EC: 2.6.1.81]; Acetylornithine transaminase. [EC: 2.6.1.81, 2.6.1.11]

Predicted SEED Role

"Succinylornithine transaminase (EC 2.6.1.81)" in subsystem Arginine and Ornithine Degradation (EC 2.6.1.81)

MetaCyc Pathways

• superpathway of arginine and polyamine biosynthesis (16/17 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• L-arginine biosynthesis II (acetyl cycle) (9/10 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• L-arginine degradation II (AST pathway) (5/5 steps found)
• L-ornithine biosynthesis I (5/5 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Lysine biosynthesis
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.11, 2.6.1.81

Use Curated BLAST to search for 2.6.1.11 or 2.6.1.81

Protein Sequence (406 amino acids)

>EX28DRAFT_1752 transaminase, acetylornithine/succinylornithine family/succinylornithine transaminase family (Enterobacter asburiae PDN3)
MSLSITRENFDEWMMPVYAPAAFIPVRGEGSRLWDQQGKEYIDFAGGIAVNALGHAHPAL
RQALNDQAAKFWHTGNGFTNEPALRLAKKLIDATFAEKVFFCNSGAEANEAALKLARKYA
HDKFGTHKSGIVAFKNAFHGRTLFTVSAGGQPSYSQDFAPLPPDIRHGIYNDLQSASELI
NDTTCAVIVEPMQGEGGVLPAQKTFLQGLRELCDRHNAVLIFDEVQTGVGRTGELYAYMH
YGVTPDVLSTAKALGGGFPIGATLTTDKFASVMTVGTHGTTYGGNPLATAVAGQVLDIIN
TPEVLKGVKQRHDWFVERLNAINGKTGLFKEIRGLGLLIGCELTAEFAGKAKLISQEAAK
VGVMVLIAGANVVRFAPALIVSEEEVQTGLDRFALACEKVKSGVSS