Protein Info for EX28DRAFT_1732 in Enterobacter asburiae PDN3

Annotation: haloacid dehalogenase superfamily, subfamily IA, variant 3 with third motif having DD or ED

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00702: Hydrolase
  • amino acids 9 to 184 (176 residues), 88.9 bits, E=6e-29
• PF13419: HAD_2
  • amino acids 11 to 191 (181 residues), 83.6 bits, E=1.9e-27
• TIGR01509: HAD hydrolase, family IA, variant 3
  • amino acids 88 to 191 (104 residues), 41.7 bits, E=6.4e-15

Best Hits

Swiss-Prot: 88% identical to HXPB_ECOLI: Hexitol phosphatase B (hxpB) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 95% identity to enc:ECL_02428)

MetaCyc: 88% identical to hexitol phosphatase B (Escherichia coli K-12 substr. MG1655)
2-deoxyglucose-6-phosphatase. [EC: 3.1.3.68]; Sorbitol-6-phosphatase. [EC: 3.1.3.68, 3.1.3.50]; Mannitol-1-phosphatase. [EC: 3.1.3.68, 3.1.3.50, 3.1.3.22]

Predicted SEED Role

"2-deoxyglucose-6-phosphate hydrolase YniC" in subsystem 2-phosphoglycolate salvage

MetaCyc Pathways

• mannitol cycle (4/5 steps found)
• 2-deoxy-D-glucose 6-phosphate degradation (3/4 steps found)
• D-sorbitol biosynthesis I (2/3 steps found)
• mannitol biosynthesis (2/3 steps found)

KEGG Metabolic Maps

• Fructose and mannose metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.3.22 or 3.1.3.50 or 3.1.3.68

Protein Sequence (223 amino acids)

>EX28DRAFT_1732 haloacid dehalogenase superfamily, subfamily IA, variant 3 with third motif having DD or ED (Enterobacter asburiae PDN3)
MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRNELPDTLGLRIDMVVDL
WFAQQPWVGPNRDEVTARIISRAISLVEENRPLLPGVREAVALCKAQGLKVGLASASPLH
MLEKVLTMFELRDSFDALASAEKLPYSKPHPQVYLDCAAKLGLDPLNCVALEDSVNGMVA
SKAARMRSIVVPAEEGRHDPRFALADVKLNSLAELTAHHLHGG