Protein Info for EX28DRAFT_1726 in Enterobacter asburiae PDN3

Annotation: threonyl-tRNA synthetase (EC 6.1.1.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 642 PF02824: TGS" amino acids 4 to 61 (58 residues), 55.3 bits, see alignment 1.1e-18 TIGR00418: threonine--tRNA ligase" amino acids 72 to 631 (560 residues), 822 bits, see alignment E=1.6e-251 PF07973: tRNA_SAD" amino acids 172 to 219 (48 residues), 59.1 bits, see alignment 7.1e-20 PF00587: tRNA-synt_2b" amino acids 318 to 528 (211 residues), 208.5 bits, see alignment E=1.7e-65 PF03129: HGTP_anticodon" amino acids 541 to 630 (90 residues), 88.2 bits, see alignment E=6.5e-29

Best Hits

Swiss-Prot: 98% identical to SYT_ENT38: Threonine--tRNA ligase (thrS) from Enterobacter sp. (strain 638)

KEGG orthology group: K01868, threonyl-tRNA synthetase [EC: 6.1.1.3] (inferred from 99% identity to enc:ECL_02421)

MetaCyc: 94% identical to threonine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Threonine--tRNA ligase. [EC: 6.1.1.3]; 6.1.1.3 [EC: 6.1.1.3]; 3.1.1.- [EC: 6.1.1.3]

Predicted SEED Role

"Threonyl-tRNA synthetase (EC 6.1.1.3)" (EC 6.1.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (642 amino acids)

>EX28DRAFT_1726 threonyl-tRNA synthetase (EC 6.1.1.3) (Enterobacter asburiae PDN3)
MPVITLPDGSQRHFDRAVSPMDVALDIGPGLAKATIAGRVNGELVDASDLIENDATLAII
TAKDEEGLEIIRHSCAHLLGHAIKQLWPNTKMAIGPVIDNGFYYDVDLDHTLTQEDIDAL
EKRMHELAETNYDVIKKKVSWHEARETFVKRGESYKVSILDENISHDDKPGLYHHEEYVD
MCRGPHVPNMRFCHHFKLMKIAGAYWRGDSNNKMLQRIYGTAWADKKALNAYLLRLEEAA
KRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRELETFVRSKLKEYQYQEVKGPFMMD
RVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNCPGHVQIFNQGLKSYRDLPLRMAEFGS
CHRNEPSGALHGLMRVRGFTQDDAHIFCTEDQVRDEVNACIRMVYDMYSTFGFEKIVVKL
STRPEKRIGSDETWDRAEADLAVALEENGIPFEYQLGEGAFYGPKIEFTLYDCLDRAWQC
GTVQLDFSLPQRLSASYVGEDNERQVPVMIHRAILGSLERFIGILTEEFAGFFPTWLAPV
QVVVMNITDSQADYVKELTQKLQNAGIRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKE
VEAGKVAVRTRRGKDLGSLDVSEVIEKLQQEIRSRSLQQLEE