Protein Info for EX28DRAFT_1658 in Enterobacter asburiae PDN3

Annotation: Cyclopropane fatty acid synthase and related methyltransferases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 382 PF02353: CMAS" amino acids 108 to 368 (261 residues), 353.5 bits, see alignment E=4e-109 PF13489: Methyltransf_23" amino acids 155 to 288 (134 residues), 53.6 bits, see alignment E=1.1e-17 PF01728: FtsJ" amino acids 155 to 236 (82 residues), 34.5 bits, see alignment E=1e-11 PF05175: MTS" amino acids 158 to 234 (77 residues), 24.6 bits, see alignment E=9.1e-09 PF01135: PCMT" amino acids 159 to 215 (57 residues), 23.8 bits, see alignment E=1.8e-08 PF07021: MetW" amino acids 165 to 232 (68 residues), 22.4 bits, see alignment E=4.3e-08 PF13847: Methyltransf_31" amino acids 166 to 269 (104 residues), 45.9 bits, see alignment E=2.7e-15 PF13649: Methyltransf_25" amino acids 171 to 261 (91 residues), 65.1 bits, see alignment E=3.8e-21 PF08241: Methyltransf_11" amino acids 172 to 264 (93 residues), 54.1 bits, see alignment E=1e-17 PF08242: Methyltransf_12" amino acids 172 to 263 (92 residues), 40.8 bits, see alignment E=1.5e-13

Best Hits

Swiss-Prot: 86% identical to CFA_ECOLI: Cyclopropane-fatty-acyl-phospholipid synthase (cfa) from Escherichia coli (strain K12)

KEGG orthology group: K00574, cyclopropane-fatty-acyl-phospholipid synthase [EC: 2.1.1.79] (inferred from 96% identity to enc:ECL_02344)

MetaCyc: 86% identical to cyclopropane fatty acyl phospholipid synthase (Escherichia coli K-12 substr. MG1655)
Cyclopropane-fatty-acyl-phospholipid synthase. [EC: 2.1.1.79]

Predicted SEED Role

"Cyclopropane-fatty-acyl-phospholipid synthase (EC 2.1.1.79)" (EC 2.1.1.79)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.79

Use Curated BLAST to search for 2.1.1.79

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (382 amino acids)

>EX28DRAFT_1658 Cyclopropane fatty acid synthase and related methyltransferases (Enterobacter asburiae PDN3)
MSSSCIEEVSVPDDNWSRIVSELLGRAGITINGSSPSDPQVKHPDFFKRVLREGSLGLGE
SYMDGWWECERLDTFFASVLRAGLENQLPRNLKDTLRVASARLFNLQSKKRAWIVGKEHY
DLGNDLFSRMLDPFMQYSCAYWKEASTLEEAQQAKLRLISEKLQLQPGMRVLDIGCGWGG
LAYFMAKHYGVSVVGVTISAEQQKMARERCQGLDVDIRLQDYRDLNEQFDRIVSVGMFEH
VGPKNYATYFEVVDRNLKPDGIFLLHTIGSKRTDNNVDPWINKYIFPNGCLPSVRQIANA
SEPHFVMEDWHNFGADYDTTLMAWHARFQETWPEIADNYSERFRRMFSYYLNACAGAFRA
RDIQLWQVVFSRGIEHGLRVAR