Protein Info for EX28DRAFT_1634 in Enterobacter asburiae PDN3

Annotation: Pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00558: pyridoxamine 5'-phosphate oxidase
  • amino acids 8 to 223 (216 residues), 332.4 bits, E=5.7e-104
• PF01243: PNPOx_N
  • amino acids 44 to 170 (127 residues), 109.6 bits, E=1.2e-35
• PF10590: PNP_phzG_C
  • amino acids 183 to 223 (41 residues), 68.8 bits, 2.9e-23

Best Hits

Swiss-Prot: 95% identical to PDXH_ENT38: Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) from Enterobacter sp. (strain 638)

KEGG orthology group: K00275, pyridoxamine 5'-phosphate oxidase [EC: 1.4.3.5] (inferred from 99% identity to enc:ECL_02317)

MetaCyc: 91% identical to pyridoxine/pyridoxamine 5'-phosphate oxidase (Escherichia coli K-12 substr. MG1655)
Pyridoxal 5'-phosphate synthase. [EC: 1.4.3.5]; 1.4.3.5 [EC: 1.4.3.5]

Predicted SEED Role

"Pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5)" in subsystem Pyridoxin (Vitamin B6) Biosynthesis (EC 1.4.3.5)

MetaCyc Pathways

• superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (12/12 steps found)
• pyridoxal 5'-phosphate biosynthesis I (7/7 steps found)
• pyridoxal 5'-phosphate salvage I (6/6 steps found)
• pyridoxal 5'-phosphate salvage II (plants) (8/9 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis II (5/8 steps found)

KEGG Metabolic Maps

• Vitamin B6 metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.3.5

Protein Sequence (223 amino acids)

>EX28DRAFT_1634 Pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5) (Enterobacter asburiae PDN3)
MSANAMSDNDELQQIAHLRREYTKGGLRRQDLPAEPLVLFERWLKQACEAKLADPTAMVV
ATVDENGQPYQRIVLLKHYDEKGLVFYTNLGSRKAHHLENNPRISLLFPWHMLERQVMVT
GKAERLSTLEVVKYFHSRPRDSQIGAWVSKQSSRISARGVLESKFLELKQKFQQGEIPLP
SFWGGFRIPIEQMEFWQGGEHRLHDRFLYQRENGGWKIDRLAP