Protein Info for EX28DRAFT_1632 in Enterobacter asburiae PDN3

Annotation: pyridoxal kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00687: pyridoxal kinase
  • amino acids 1 to 285 (285 residues), 461.7 bits, E=4.7e-143
• PF08543: Phos_pyr_kin
  • amino acids 74 to 261 (188 residues), 58.7 bits, E=5.9e-20
• PF00294: PfkB
  • amino acids 131 to 248 (118 residues), 40.4 bits, E=2.4e-14

Best Hits

Swiss-Prot: 90% identical to PDXY_ECO57: Pyridoxal kinase PdxY (pdxY) from Escherichia coli O157:H7

KEGG orthology group: K00868, pyridoxine kinase [EC: 2.7.1.35] (inferred from 94% identity to enc:ECL_02315)

MetaCyc: 90% identical to pyridoxal kinase 2 (Escherichia coli K-12 substr. MG1655)
Pyridoxal kinase. [EC: 2.7.1.35]

Predicted SEED Role

"Pyridoxal kinase (EC 2.7.1.35)" in subsystem Pyridoxin (Vitamin B6) Biosynthesis (EC 2.7.1.35)

MetaCyc Pathways

• superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (12/12 steps found)
• pyridoxal 5'-phosphate salvage I (6/6 steps found)
• pyridoxal 5'-phosphate salvage II (plants) (8/9 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis II (5/8 steps found)

KEGG Metabolic Maps

• Vitamin B6 metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.35

Use Curated BLAST to search for 2.7.1.35

Protein Sequence (286 amino acids)

>EX28DRAFT_1632 pyridoxal kinase (Enterobacter asburiae PDN3)
MKNILAIQSHVVFGHAGNSAAEFPMRRLGVNVWPLNTVQFSNHTQYGKWTGCVMPPSHLT
EVVQGIADIDQLKRCDAVLSGYLGSAEQGEHILGIVRRVKAANPSAKYFCDPVMGHPEKG
CIVAPGVAEFHVRHALPASDIIAPNLVELEILCERPVNSVEEAVSASRELIAQGPKVVLV
KHLARAGLSQDRFEMLLVTKDDAWHISRPLVDFGLRQPVGVGDVTSGLLLVKLLQGATMR
DALEHVTAAVYEIMIATKQMQEYELQVVAAQDRIAKPEHYFSATRL