Protein Info for EX28DRAFT_1628 in Enterobacter asburiae PDN3

Annotation: electron transport complex, RnfABCDGE type, E subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 228 transmembrane" amino acids 19 to 32 (14 residues), see Phobius details amino acids 38 to 59 (22 residues), see Phobius details amino acids 71 to 91 (21 residues), see Phobius details amino acids 97 to 116 (20 residues), see Phobius details amino acids 127 to 148 (22 residues), see Phobius details amino acids 183 to 206 (24 residues), see Phobius details TIGR01948: electron transport complex, RnfABCDGE type, E subunit" amino acids 6 to 209 (204 residues), 273.5 bits, see alignment E=4.5e-86 PF02508: Rnf-Nqr" amino acids 6 to 199 (194 residues), 212.8 bits, see alignment E=1.9e-67

Best Hits

Swiss-Prot: 91% identical to RNFE_KLEP3: Ion-translocating oxidoreductase complex subunit E (rnfE) from Klebsiella pneumoniae (strain 342)

KEGG orthology group: K03613, electron transport complex protein RnfE (inferred from 96% identity to enc:ECL_02311)

Predicted SEED Role

"Electron transport complex protein RnfE" in subsystem Na(+)-translocating NADH-quinone oxidoreductase and rnf-like group of electron transport complexes

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (228 amino acids)

>EX28DRAFT_1628 electron transport complex, RnfABCDGE type, E subunit (Enterobacter asburiae PDN3)
MSQVKEVIVQGLWKNNSALVQLLGMCPLLAVTSTATNALGLGLATTLVLTLTNLSISALR
RWTPSEIRIPIYVMIIASVVSIVQMLINAYAFGLYQSLGIFIPLIVTNCIVVGRAEAFAV
KNSPAMSALDGFSIGMGATCAMFVLGSLREILGNGTLFDGADALLGGWAKALRVEVFHTD
TPFLLAMLPPGAFIGLGMMLAIKYLIDEKRKRRAAERSVLEGTPEKAS