Protein Info for EX28DRAFT_1509 in Enterobacter asburiae PDN3

Annotation: Membrane transporters of cations and cationic drugs

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 120 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details transmembrane" amino acids 30 to 51 (22 residues), see Phobius details amino acids 58 to 79 (22 residues), see Phobius details amino acids 85 to 103 (19 residues), see Phobius details PF00893: Multi_Drug_Res" amino acids 3 to 93 (91 residues), 96 bits, see alignment E=8e-32

Best Hits

Swiss-Prot: 89% identical to MDTJ_ENT38: Spermidine export protein MdtJ (mdtJ) from Enterobacter sp. (strain 638)

KEGG orthology group: K11743, spermidine export protein MdtJ (inferred from 92% identity to enc:ECL_02236)

MetaCyc: 84% identical to multidrug/spermidine efflux pump membrane subunit MdtJ (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-350; TRANS-RXN0-266

Predicted SEED Role

"Spermidine export protein MdtJ"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (120 amino acids)

>EX28DRAFT_1509 Membrane transporters of cations and cationic drugs (Enterobacter asburiae PDN3)
MFYWILLALAIIAEITGTLSMKWASVSDGNTGFILMLVMISLSYIFLSFAVKKIALGVAY
ALWEGIGILLITLFSVLIFDETLTTMKVAGLTTLVAGIVLIKSGTRKPGKQQKEQNHATV