Protein Info for EX28DRAFT_1457 in Enterobacter asburiae PDN3

Annotation: L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 404 PF02746: MR_MLE_N" amino acids 13 to 110 (98 residues), 120.6 bits, see alignment E=4.2e-39 PF13378: MR_MLE_C" amino acids 139 to 379 (241 residues), 155.5 bits, see alignment E=1.8e-49

Best Hits

Swiss-Prot: 96% identical to MAND_ENT38: D-galactonate dehydratase family member Ent638_1932 (Ent638_1932) from Enterobacter sp. (strain 638)

KEGG orthology group: K08323, starvation sensing protein RspA (inferred from 98% identity to enc:ECL_02183)

Predicted SEED Role

"Starvation sensing protein RspA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (404 amino acids)

>EX28DRAFT_1457 L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily (Enterobacter asburiae PDN3)
MKIVAADVFVTCPGRNFVTLKITTDEGIVGLGDATLNGRELSVASYLKDHLCPQLIGRDA
HRIEDVWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAANMPLYQLLGGASREGVMVY
CHTTGHTINDVLEDYARHKEMGFKAIRVQCGVPGMKTTYGMAKGKGLAYEPATKGDWPEE
QLWSTEKYLDFTPKLFDAVRSKFGFNEHLLHDMHHRLTPIEAARFGKSIEEFRMFWMEDP
TPAENQACFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLIDYIRTTITHAGGITGMRRI
ADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWVPNFGVQEYMGYSEQMLEVFPHSWRFD
NGYMHPGDKPGLGIEFDEKLAAKYPYDPAYLPVARLEDGTLWNW