Protein Info for EX28DRAFT_1450 in Enterobacter asburiae PDN3

Annotation: Short-chain alcohol dehydrogenase of unknown specificity

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00106: adh_short
  • amino acids 2 to 187 (186 residues), 184.3 bits, E=4.6e-58
• PF08659: KR
  • amino acids 3 to 171 (169 residues), 53.3 bits, E=8.8e-18
• PF01370: Epimerase
  • amino acids 3 to 134 (132 residues), 25.2 bits, E=2.6e-09
• PF13561: adh_short_C2
  • amino acids 7 to 220 (214 residues), 127.1 bits, E=2.2e-40
• PF03807: F420_oxidored
  • amino acids 12 to 51 (40 residues), 22.6 bits, 3.4e-08

Best Hits

Swiss-Prot: 90% identical to YDFG_SALTI: NADP-dependent 3-hydroxy acid dehydrogenase YdfG (ydfG) from Salmonella typhi

KEGG orthology group: K00540, [EC: 1.-.-.-] (inferred from 96% identity to enc:ECL_02171)

MetaCyc: 88% identical to 3-hydroxy acid dehydrogenase YdfG (Escherichia coli K-12 substr. MG1655)
RXN-16000 [EC: 1.1.1.381]; RXN-8974 [EC: 1.1.1.381, 1.1.1.298]; Serine 3-dehydrogenase. [EC: 1.1.1.381, 1.1.1.298, 1.1.1.276]

Predicted SEED Role

"3-hydroxypropionate dehydrogenase (EC 1.1.1.298)" (EC 1.1.1.298)

MetaCyc Pathways

• superpathway of L-threonine metabolism (16/18 steps found)
• uracil degradation III (5/5 steps found)
• L-threonine degradation III (to methylglyoxal) (3/3 steps found)
• aminopropanol phosphate biosynthesis II (3/4 steps found)
• β-alanine degradation III (1/2 steps found)
• 3-hydroxypropanoate cycle (6/13 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (9/18 steps found)
• glyoxylate assimilation (5/13 steps found)
• superpathway of the 3-hydroxypropanoate cycle (6/18 steps found)

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Carotenoid biosynthesis - General
• Insect hormone biosynthesis
• Nucleotide sugars metabolism
• Porphyrin and chlorophyll metabolism
• Puromycin biosynthesis
• Trinitrotoluene degradation
• alpha-Linolenic acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.- or 1.1.1.276 or 1.1.1.298 or 1.1.1.381

Protein Sequence (248 amino acids)

>EX28DRAFT_1450 Short-chain alcohol dehydrogenase of unknown specificity (Enterobacter asburiae PDN3)
MIILVTGATAGFGESITRRFVANGHKVIATGRRQERLQELKDELGDSILTAQLDVRNRAA
IEEMIANLPAEWRAIDVLVNNAGLALGMEPAHKASVEDWENMIDTNNKGLVYMTRAVLPG
MVERNRGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLNLRTDLHGTAIRVTDIEPGLV
GGTEFSNVRFKGDDAKAGKTYENANALTPEDVTETVWWVATLPKHVNINTVEMMPVSQSF
AGLSVHRG