Protein Info for EX28DRAFT_1422 in Enterobacter asburiae PDN3

Annotation: Cu2+-containing amine oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 758 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details PF07833: Cu_amine_oxidN1" amino acids 34 to 110 (77 residues), 50.2 bits, see alignment E=5.8e-17 PF02727: Cu_amine_oxidN2" amino acids 124 to 209 (86 residues), 80.8 bits, see alignment E=1.7e-26 PF21994: AGAO-like_N2" amino acids 130 to 190 (61 residues), 29.9 bits, see alignment 1.3e-10 PF02728: Cu_amine_oxidN3" amino acids 216 to 316 (101 residues), 90.2 bits, see alignment E=2.3e-29 PF01179: Cu_amine_oxid" amino acids 348 to 754 (407 residues), 382 bits, see alignment E=8.1e-118

Best Hits

Swiss-Prot: 86% identical to AMO_KLEOK: Primary amine oxidase (maoA) from Klebsiella oxytoca (strain ATCC 8724 / DSM 4798 / JCM 20051 / NBRC 3318 / NRRL B-199 / KCTC 1686)

KEGG orthology group: K00276, primary-amine oxidase [EC: 1.4.3.21] (inferred from 94% identity to enc:ECL_02157)

MetaCyc: 84% identical to copper-containing amine oxidase (Escherichia coli K-12 substr. MG1655)
Primary-amine oxidase. [EC: 1.4.3.21, 1.4.3.4]; 1.4.3.21 [EC: 1.4.3.21, 1.4.3.4]; 1.4.3.21 [EC: 1.4.3.21, 1.4.3.4]; 1.4.3.21 [EC: 1.4.3.21, 1.4.3.4]

Predicted SEED Role

"Monoamine oxidase (1.4.3.4)" in subsystem Aromatic Amin Catabolism or Auxin biosynthesis or Glycine and Serine Utilization or Threonine degradation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.3.21 or 1.4.3.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (758 amino acids)

>EX28DRAFT_1422 Cu2+-containing amine oxidase (Enterobacter asburiae PDN3)
MGSNSSFSARRTALAMAVALCCAWQSPVFAHGSEAHMVPMDKTLQDFGADVQWDDYAQMF
TIAKDGAFVKVKPGAKTAIVNGKTLRLEVPVVMKGKKAFISDTFINDVFQSGLDQTFQVE
KRPHPLNALTADEIKQAVEIVKASADFKPNTRFTQIALAEPEKAKVWDFVLNGTAVDAPR
QAKIVMLDGKHVIESVVDLKDKKILRWEPVKDAHGMVLLDDFTAVQQIINESTEFAEVLK
KHGITDPKKVITTPLTVGYFDGKDGLKQEDRLLKVVSYLDVGDGNYWAHPIENLVAVVDL
EQKKIQKIEEGAVVPVPMTPRPYDGRDRVETPKKPLNITEPEGKNYTITGDMVHWQNWDF
HLSLDSRVGPMISTVTYNDNGKKRQIMYQGSLGGMIVPYGDPDVGWYFKAYLDSGDYGMG
TLTSPLVRGKDVPSNAVMLNETIPDYTGAPMEIPRAIAIFEGYAGPEYKHQEMGQPNVST
ERRELVVRWVSTVGNYDYIFDWVFHENGTIGIDAGATGIEAVKGVQAKTMHDATAKDDTK
YGTLIDHNIVGTTHQHIYNFRLDMDVDGINNKLVAMDPEVKPNTAGGPRTSTMQINQYDI
DTEQQAAQKFDPGTIRLLSNTSKENRMGNPVSYQIIPYAGGTHPVATGAKFAPDEWIYHR
LSFMDKQLWVTRYHPDELYPEGKFPNRSIHDTGLGQYSKDNESLNDQDDVVWMTTGTTHV
ARAEEWPIMPTEWVHTLLKPWNFFDETPTLGKKKDEQK