Protein Info for EX28DRAFT_1343 in Enterobacter asburiae PDN3

Annotation: type I secretion membrane fusion protein, HlyD family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 391 transmembrane" amino acids 21 to 40 (20 residues), see Phobius details TIGR01843: type I secretion membrane fusion protein, HlyD family" amino acids 192 to 391 (200 residues), 214.5 bits, see alignment E=1.4e-67 PF26002: Beta-barrel_AprE" amino acids 277 to 369 (93 residues), 86.8 bits, see alignment E=8.6e-29

Best Hits

KEGG orthology group: K02022, (no description) (inferred from 96% identity to enc:ECL_02073)

Predicted SEED Role

"Type I secretion system, membrane fusion protein LapC"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (391 amino acids)

>EX28DRAFT_1343 type I secretion membrane fusion protein, HlyD family (Enterobacter asburiae PDN3)
MNDFSRINSRLKEPRLPRSSLVAWSLFALLAVFIAWASLFHLDEVTTGSGKVIPSSHEQV
IQSLEGGIIHSLMVREGDIVERGQQLAQLDRTKTESSVLESESRLNAALATAARLKAEVN
DTELAFPEELDDDVELVKQETALYQSRRESLEKGLAGLRQGAELVQRELSLTRPLVTQGA
ASKVEVLRLERQKNELENKITEMKNQYYVRAREELAKANAEIEAQRSVMKGREDSLTRLT
FNAPVRGIVKDIDVTTVGGVIPPNGKLMSLVPLDDQMVVEAKISPRDVAFIHPGQKALVK
ITAYDYSIYGGLEGEVTMISPDTLQDEVKRDVYYYRVYIRTDSNHLTNKQGQEFPVFPGM
IATVDIKTGSKTILDYLLKPLNKAKEALRER