Protein Info for EX28DRAFT_1261 in Enterobacter asburiae PDN3

Annotation: Nucleotidyltransferase/DNA polymerase involved in DNA repair

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 422 PF00817: IMS" amino acids 5 to 150 (146 residues), 143.5 bits, see alignment E=7.3e-46 PF11799: IMS_C" amino acids 245 to 360 (116 residues), 85.9 bits, see alignment E=3.7e-28 PF13438: DUF4113" amino acids 372 to 421 (50 residues), 77.4 bits, see alignment 1e-25

Best Hits

Swiss-Prot: 91% identical to UMUC_SALTY: Protein UmuC (umuC) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03502, DNA polymerase V (inferred from 96% identity to ent:Ent638_2212)

MetaCyc: 84% identical to DNA polymerase V catalytic protein (Escherichia coli K-12 substr. MG1655)
DNA-directed DNA polymerase. [EC: 2.7.7.7]

Predicted SEED Role

"Error-prone, lesion bypass DNA polymerase V (UmuC)"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (422 amino acids)

>EX28DRAFT_1261 Nucleotidyltransferase/DNA polymerase involved in DNA repair (Enterobacter asburiae PDN3)
MFALCDVNSFYASCETVFRPDLRGRPVVVLSNNDGCVIARSAEAKAAGITMGEPFFKQKE
LFRRAGVVCFSSNYELYADMSNRVMTTLEEMSPRVEIYSIDEAFCDLTGVRNCRDLTEFG
KEIRATVLKRTHLTVGVGIAQTKTLAKLANHAAKKWQRQTGGVVDLSNIYRQRRLLALVP
VEYVWGVGRRISKKLNAMGIKTALDLSEQSTWIIRKHFNVVLERTVRELRGEPCLDLEEF
APAKQEIVCSRSFGERVTEYEQMRQAICSYAARGAEKLRGEHQYCRFISAFVKTSPFALN
EPYYGNSASMKLLTPTQDSRDIINAAVKCLDKIWKDGHRYQKAGIMLGDFFSQGVAQLNL
FDENAPRVGSDKLMEVLDHLNAKEGKGTLYFAGQGVQQQWQMKREMLSPRYTTRYSDLLI
VK