Protein Info for EX28DRAFT_1247 in Enterobacter asburiae PDN3

Annotation: Glycosidases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00128: Alpha-amylase
  • amino acids 12 to 449 (438 residues), 253 bits, E=1.1e-78
• PF22157: SupH-like_C
  • amino acids 478 to 533 (56 residues), 29.2 bits, 1.7e-10

Best Hits

KEGG orthology group: K05343, maltose alpha-D-glucosyltransferase [EC: 5.4.99.16] (inferred from 90% identity to enc:ECL_02049)

Predicted SEED Role

"Trehalose synthase (EC 5.4.99.16)" in subsystem Trehalose Biosynthesis (EC 5.4.99.16)

MetaCyc Pathways

• trehalose biosynthesis IV (1/1 steps found)
• glycogen biosynthesis III (from α-maltose 1-phosphate) (4/8 steps found)

KEGG Metabolic Maps

• Starch and sucrose metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.99.16

Protein Sequence (541 amino acids)

>EX28DRAFT_1247 Glycosidases (Enterobacter asburiae PDN3)
MTDWHTRAIIYQIDTALFYDLNGDGCGDIAGIAAKLRYIRRMGATVIWITPFYLTPFLDE
GYDVSDHLQVDPRFGKLADIIAFIEQARELGMQVIIELLIQHTSDAHPWFQQARRSPASP
YRDYYLWSDSDEDDTPPMFPGVEKSIWTWDDEAGQYYRHMFYRHEPDLNLTSPAVLKEIK
NIILFWLKLGVSGFRLDAASHLTTQAGNGDEKKGLWILEHMRRLIEERNPDAILLGEVDV
DVETYKDYFGKNDRLNLVLNFWLNKYFYVSLARKSARPLRNAVKKMIVPPDACCFANWLR
NHDELDLEGISQKDKQFVLDTFAPDEEMNVYQRGIRRRLAPMLNGDRKRLAFCHAVLFSL
PGVPVMRYGDEIGMGDDLELEERYAVRTPMQWAGSQGGGFSAADPEKFIAPIIDHGPHRY
QKVNVADSLLHHNSLLHRIIDIANTRSEFPEIAVAPFRLITIDDDAVLGLYYETDERSIL
TFVNFSDQPVQFTTKGIRNATWTACLEDKRYDDALICGKTVQLTLGGYGYRWFWTHRSAL
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