Protein Info for EX28DRAFT_1130 in Enterobacter asburiae PDN3

Annotation: RNA polymerase sigma factor, sigma-70 family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 186 TIGR02937: RNA polymerase sigma factor, sigma-70 family" amino acids 18 to 176 (159 residues), 68.9 bits, see alignment E=2e-23 PF04542: Sigma70_r2" amino acids 18 to 77 (60 residues), 32.5 bits, see alignment E=5.8e-12 PF08281: Sigma70_r4_2" amino acids 120 to 170 (51 residues), 37.4 bits, see alignment E=1.6e-13

Best Hits

KEGG orthology group: None (inferred from 88% identity to enc:ECL_01937)

Predicted SEED Role

"RNA polymerase sigma-54 factor RpoN" in subsystem Flagellar motility or Flagellum or Transcription initiation, bacterial sigma factors

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (186 amino acids)

>EX28DRAFT_1130 RNA polymerase sigma factor, sigma-70 family (Enterobacter asburiae PDN3)
MKAGEAGGSMLMSALNACRARLTAFIRGRTSVRDDADDILQEVTYQLMKVEQPVENVAAW
LFRAARNEMTDRARKKREVSLSGYFTGVDEEGFPEDELAETLFGVPHTPEEEYLKTLLWE
ELGQALSELPPPQREVFEKTELHGYSIKMLAEETGASEQALLSRKHKAVLFLRTRLRDVY
DALTGV