Protein Info for EX28DRAFT_1124 in Enterobacter asburiae PDN3

Annotation: sucrose-6-phosphate hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01322: sucrose-6-phosphate hydrolase
  • amino acids 15 to 442 (428 residues), 554.5 bits, E=8.4e-171
• PF00251: Glyco_hydro_32N
  • amino acids 31 to 336 (306 residues), 341.7 bits, E=5.1e-106
• PF08244: Glyco_hydro_32C
  • amino acids 363 to 444 (82 residues), 39.8 bits, E=4.8e-14

Best Hits

Swiss-Prot: 69% identical to SCRB_KLEPN: Sucrose-6-phosphate hydrolase (scrB) from Klebsiella pneumoniae

KEGG orthology group: K01193, beta-fructofuranosidase [EC: 3.2.1.26] (inferred from 88% identity to enc:ECL_01932)

MetaCyc: 69% identical to sucrose-6-phosphate hydrolase (Klebsiella pneumoniae)
3.2.1.-

Predicted SEED Role

"Sucrose-6-phosphate hydrolase (EC 3.2.1.B3)" (EC 3.2.1.B3)

MetaCyc Pathways

• sucrose degradation III (sucrose invertase) (4/4 steps found)
• sucrose degradation I (sucrose phosphotransferase) (3/3 steps found)
• sucrose degradation V (sucrose α-glucosidase) (3/5 steps found)
• inulin degradation (1/3 steps found)

KEGG Metabolic Maps

• Galactose metabolism
• Starch and sucrose metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.26, 3.2.1.B3

Use Curated BLAST to search for 3.2.1.26 or 3.2.1.B3

Protein Sequence (468 amino acids)

>EX28DRAFT_1124 sucrose-6-phosphate hydrolase (Enterobacter asburiae PDN3)
MTLPSRWPAILQAVMKGQPRALADSHYPQWHPAPVTGLMNDPNGFIWFAGRYHLFYQWNP
LDCEHRFKCWGHWSSADLVHWRHEPLALMPDEEYDRNGCYSGSAVDNHGLLTLCYTGNVK
FDDGSRTAWQCLAVEQPDGTFKKLGPVLALPEGYTGHVRDPKVWQHDGEWYMVLGAQDVQ
KRGKVLLFKSADLHAWTSCGEIAGHGVNGLTDAGYMWECPDLFALDGTHILISCPQGLAR
EPHRYLNTYPAAWMSGAFDYASAAFHHGDLHELDAGFEFYAPQTTLAEDGRRILIGWMGV
PDGEEMLQPTRAHGWMHQMTCPRELRYRDGRLWQTPARELAALREDEQRWQGRASEAPDL
DATRLEFELSASQVNVDFGGALRLTVDEQGIRLERASLKTGETLTRYWQGNVTHLRVLCD
RSSVEIFINHGEGVMSSRYFPDHPARVRFEGASDITLRYWSLRACMIE