Protein Info for EX28DRAFT_1074 in Enterobacter asburiae PDN3

Annotation: ABC-type branched-chain amino acid transport systems, periplasmic component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 385 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF13458: Peripla_BP_6" amino acids 30 to 372 (343 residues), 212.2 bits, see alignment E=2.7e-66 PF01094: ANF_receptor" amino acids 51 to 369 (319 residues), 97.2 bits, see alignment E=1.5e-31 PF13433: Peripla_BP_5" amino acids 161 to 357 (197 residues), 32.5 bits, see alignment E=7.5e-12

Best Hits

KEGG orthology group: K01999, branched-chain amino acid transport system substrate-binding protein (inferred from 95% identity to cko:CKO_02937)

Predicted SEED Role

"Leucine-, isoleucine-, valine-, threonine-, and alanine-binding protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (385 amino acids)

>EX28DRAFT_1074 ABC-type branched-chain amino acid transport systems, periplasmic component (Enterobacter asburiae PDN3)
MKTVKISALSAAILLGSFASTAAWAADGETVVIGLAGPLTGPSARIGKDLENGAQLAIDD
INKQHPTIGGKAVTFKLQSEDDQSDPRTAVAVAQRLVDSGVAGVVGHWNTGTSIPAARIY
HDAGIAQVAPVATGHAYTKQGFDTSFRVMGHDDDGGQLAGQYAVNTLKAKRIAVIDDRTA
FGQGLADQFIKSLEAQGVKVVDRQYVDDKTVDFSAVLTAIRSKNADLIFFGGVDSQAAPL
ARRIKQLGMNATLMGAGGFVSQTFLQLAQKEGEGVVALEPGLPVEQMPGGKAFEQAYQSR
YHTHIELHAPFAYDATRVLVAAMEKADSVEPSDYLPALRAISYSGVTGQIAFDKEGNLKS
PSFTVYKVVDGKWQPQTVLGGAKAK