Protein Info for EX28DRAFT_1007 in Enterobacter asburiae PDN3

Annotation: Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 311 PF01171: ATP_bind_3" amino acids 42 to 205 (164 residues), 52.4 bits, see alignment E=2.9e-18

Best Hits

Swiss-Prot: 94% identical to TTCA_CITK8: tRNA-cytidine(32) 2-sulfurtransferase (ttcA) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)

KEGG orthology group: K14058, tRNA 2-thiocytidine biosynthesis protein TtcA (inferred from 97% identity to enc:ECL_01830)

MetaCyc: 93% identical to tRNA cytosine32 2-sulfurtransferase TtcA (Escherichia coli K-12 substr. MG1655)
2.8.1.M2 [EC: 2.8.1.M2]

Predicted SEED Role

"tRNA(Cytosine32)-2-thiocytidine synthetase"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.M2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (311 amino acids)

>EX28DRAFT_1007 Predicted ATPase of the PP-loop superfamily implicated in cell cycle control (Enterobacter asburiae PDN3)
MQENQEINKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMVCLSGGKDSYTMLEILR
NLQQSAPVKFSLVAVNLDQKQPGFPEHILPEYLEKLGVEYKIVEENTYGIVKEKIPEGKT
TCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFLNMFYGGKMKGMPPKLMSDDG
KHIVIRPLAYCREKDIERFSQAKGFPIIPCNLCGSQPNLQRQVIGDMLRDWDKRYPGRIE
TMFSAMQNVVPSHLADVELFDFKGINHESEVVNGGDLAFDREDIPMQPAGWQPEEDDNQF
EELRLNVLEVK