Protein Info for EX28DRAFT_0935 in Enterobacter asburiae PDN3

Annotation: glutamine amidotransferase of anthranilate synthase or aminodeoxychorismate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00566: glutamine amidotransferase of anthranilate synthase or aminodeoxychorismate synthase
  • amino acids 4 to 187 (184 residues), 183.3 bits, E=4.3e-58
• PF00117: GATase
  • amino acids 5 to 186 (182 residues), 174.3 bits, E=4.6e-55
• PF07722: Peptidase_C26
  • amino acids 74 to 172 (99 residues), 23.8 bits, E=7.2e-09
• PF02885: Glycos_trans_3N
  • amino acids 201 to 263 (63 residues), 61 bits, E=1.5e-20
• TIGR01245: anthranilate phosphoribosyltransferase
  • amino acids 204 to 528 (325 residues), 423.6 bits, E=6e-131
• PF00591: Glycos_transf_3
  • amino acids 271 to 521 (251 residues), 337.7 bits, E=1e-104

Best Hits

Swiss-Prot: 92% identical to TRPGD_ECOLI: Bifunctional protein TrpGD (trpGD) from Escherichia coli (strain K12)

KEGG orthology group: K13497, anthranilate synthase/phosphoribosyltransferase [EC: 2.4.2.18 4.1.3.27] (inferred from 99% identity to enc:ECL_01728)

MetaCyc: 92% identical to anthranilate synthase subunit TrpD (Escherichia coli K-12 substr. MG1655)
Anthranilate synthase. [EC: 4.1.3.27]; Anthranilate phosphoribosyltransferase. [EC: 4.1.3.27, 2.4.2.18]

Predicted SEED Role

"Anthranilate synthase, amidotransferase component (EC 4.1.3.27) / Anthranilate phosphoribosyltransferase (EC 2.4.2.18)" in subsystem Auxin biosynthesis or Tryptophan synthesis or Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more. (EC 2.4.2.18, EC 4.1.3.27)

MetaCyc Pathways

• superpathway of chorismate metabolism (54/59 steps found)
• superpathway of aromatic amino acid biosynthesis (18/18 steps found)
• superpathway of L-tryptophan biosynthesis (13/13 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (11/12 steps found)
• superpathway of tetrahydrofolate biosynthesis (9/10 steps found)
• L-tryptophan biosynthesis (6/6 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• 4-aminobenzoate biosynthesis I (2/2 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• 4-hydroxy-2(1H)-quinolone biosynthesis (2/5 steps found)
• acridone alkaloid biosynthesis (1/4 steps found)
• superpathway of candicidin biosynthesis (4/11 steps found)
• superpathway of quinolone and alkylquinolone biosynthesis (2/10 steps found)
• chloramphenicol biosynthesis (1/9 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Phenylalanine, tyrosine and tryptophan biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.18, 4.1.3.27

Use Curated BLAST to search for 2.4.2.18 or 4.1.3.27

Protein Sequence (531 amino acids)

>EX28DRAFT_0935 glutamine amidotransferase of anthranilate synthase or aminodeoxychorismate synthase (Enterobacter asburiae PDN3)
MADILLLDNIDSFTYNLADQLRANGHNVVIYRNHVPAQTLIDRLATMQNPVLMLSPGPGA
PSEAGCMPELLTRMRGKLPIVGICLGHQAIVEAYGGYVGQAGEILHGKASSIEHDGQAMF
AGLPNPLPVARYHSLVGSNIPAGLTINASFEGMVMAVRHDADRVCGLQFHPESILTSHGA
RLLEQTLDWALQKLEQTNTLQPILEKLYQAQTLSQQESHQLFSAVVRGELKPEQLAAALV
SMKVRGESPQEIAGAATALLENAAPFPRPDYQFADIVGTGGDGSNSINISTASAFVAAAC
GLKVAKHGNRSVSSRSGSSDLLAAFGINLDMNAERSREALDDLGVCFLFAPKYHTGFRHA
MPVRQQLKTRTLFNVLGPLINPAHPPLALIGVYSPELVLPIAETLRVLGYQRAAVVHSGG
MDEVSLHAPTLVAELRDGEILSYQLEAADFGLAPYHQEALAGGTPEENRDILTRLLQGKG
EVAHEAAVAANVAMLMRLHGEEDLKANVQKVLDVLRSGAAYDRVTALAARG