Protein Info for EX28DRAFT_0922 in Enterobacter asburiae PDN3

Annotation: TonB family C-terminal domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 245 transmembrane" amino acids 14 to 35 (22 residues), see Phobius details PF16031: TonB_N" amino acids 38 to 163 (126 residues), 74.3 bits, see alignment E=1.6e-24 PF03544: TonB_C" amino acids 166 to 235 (70 residues), 65.4 bits, see alignment E=5.3e-22 TIGR01352: TonB family C-terminal domain" amino acids 167 to 235 (69 residues), 68.4 bits, see alignment E=2.6e-23

Best Hits

Swiss-Prot: 79% identical to TONB_SALTY: Protein TonB (tonB) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03832, periplasmic protein TonB (inferred from 92% identity to enc:ECL_01647)

MetaCyc: 76% identical to Ton complex subunit TonB (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Ferric siderophore transport system, periplasmic binding protein TonB" in subsystem Campylobacter Iron Metabolism or Hemin transport system or Ton and Tol transport systems

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (245 amino acids)

>EX28DRAFT_0922 TonB family C-terminal domain (Enterobacter asburiae PDN3)
MIMTSMTLDLPRRFPWPTLLSVVIHGAVVAGLLYTSVHQVIEMPAPAQPISVTMVSPADL
EPPQVAPPPPQPVAEPEPEHEPVPPPPKEAPVVIHKPEPKPKPKPKPKPVKKVEERPKRE
ERPVEPRATQTVENAAPSRPVMNNTATAPKPTVSAPAGPRALSRNQPQYPARAQALRIEG
RVRVKFDVTADGRVDNVEILSAQPSNMFEREVKSAMRRWRYEAGKPGSGLIVNIVFRLNG
GAQME