Protein Info for EX28DRAFT_0845 in Enterobacter asburiae PDN3

Annotation: nitrite reductase [NAD(P)H], large subunit/nitrite reductase [NAD(P)H], small subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1328 PF07992: Pyr_redox_2" amino acids 2 to 272 (271 residues), 197.8 bits, see alignment E=1.4e-61 amino acids 402 to 696 (295 residues), 177.6 bits, see alignment E=1.9e-55 PF13738: Pyr_redox_3" amino acids 80 to 265 (186 residues), 37.8 bits, see alignment E=7.1e-13 PF00070: Pyr_redox" amino acids 141 to 211 (71 residues), 56.9 bits, see alignment 1.3e-18 amino acids 544 to 617 (74 residues), 57.5 bits, see alignment 8e-19 PF18267: Rubredoxin_C" amino acids 308 to 370 (63 residues), 41.4 bits, see alignment 5.9e-14 amino acids 714 to 783 (70 residues), 35.3 bits, see alignment 4.5e-12 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 402 to 1195 (794 residues), 1110.9 bits, see alignment E=0 PF04324: Fer2_BFD" amino acids 818 to 864 (47 residues), 38.9 bits, see alignment (E = 4.6e-13) PF03460: NIR_SIR_ferr" amino acids 954 to 1016 (63 residues), 45.5 bits, see alignment 2.5e-15 PF01077: NIR_SIR" amino acids 1026 to 1162 (137 residues), 79.5 bits, see alignment E=9.7e-26 PF13806: Rieske_2" amino acids 1215 to 1316 (102 residues), 93.5 bits, see alignment 3.8e-30 TIGR02378: nitrite reductase [NAD(P)H], small subunit" amino acids 1216 to 1316 (101 residues), 104.7 bits, see alignment 2.6e-34

Best Hits

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1328 amino acids)

>EX28DRAFT_0845 nitrite reductase [NAD(P)H], large subunit/nitrite reductase [NAD(P)H], small subunit (Enterobacter asburiae PDN3)
MRLVIIGNGMAATRLIASLTGRAAGRFAITVIGDEPEHAYNRIQLSPVLGGEKQAADICL
QDDDWYQARGVTVLRGEKAIAVNVDAREVQTTARTLGWDALVFATGSTPFVPPVPGCDAP
HVFTFRTLEDTRAIRSIAGPAVVLGGGVLGVETAAALARLGDNVTLVHRGPWLMEQQLDR
QAGMLLEEALAARGVRCELASGIAAVSGNSVTLLNGRRVAAARVVLATGVQPSIALAQAS
GIRCARGIVVDYQMQTSEPNVYAIGECCEIDGQTFGLVAPCLAQAEILAARLAGDATAPF
TQTDSGMRLKVTGMELFSLGRAAEQEGDMVWSAWDPLTRHYRRLLIHQGALAGALLMGDC
RSAATFTDLLATAAPAHADWLFDRFTTQPQVAGQNAMTKPTLVVVGHGMVGHHFLEDCVK
RNLHQQYQIVVFGEERYAVYDRVHLSEYFGGRSAASLSLVEGDFFADSGIELRLSQQIVA
IDRDARVVRTASGHETHWDKLVLATGSYPFVPPVPGNDLPGCFVYRTLDDLDKIAAHAAG
SRRGVVIGGGLLGLEAANALKQLGLETHVVEFAPNLMAVQLDNDGAAMLRKKTEALGVGV
HTSKATTEIATADGGLVLRFADGEQLETDMVVFSAGIRPQDSLARSSGLAIGERGGICID
DGCQTSDPDVLAIGECALWEGKIFGLVAPGYQMARVAAAALAGEDKTFTGADMSTKLKLL
GVDVASFGDAHGRTPGALSYQWTHGPQQIYKKIVVSHDGKTLLGGVLVGDASEYATLVQM
MLNGISLPKEPETLILPASSGSAPKALGVAALPESAQICSCHNVSKGDICRAVSAGAADI
GAVKQCTKAATGCGGCSALVKQVMEFQLAEQGVEVKKDICEHFPYSRQEIYHLVRVNHIR
TFDQLISRYGQGHGCEICKPLVGSVLASCWNEYLLKPAHLPLQDTNDRYFANIQKDGTYS
IVPRMPAGEVTADGLIAIGQIAKRYSLYSKITGGQRIDLFGATLEQLPAIWQALVEAGFE
TGHAYGKSLRTVKSCVGSTWCRYGVQDSTGLAVRLEHRYKGLRAPHKIKMAVSGCTRECA
EAQSKDVGVIATDKGWNLYLCGNGGMKPRHADLFASDLDDETLIRTVDRFLMFYIRTADR
LQRTSTWMDNLEGGLDYLREVILNDSLGIAHELEQEMARVVETYQCEWQTTLSDPSRLAL
FRTTVNETAPDESKRWQEICGIDDIPEQAGIGARLGRKPIALFRFGKTVYALDDREPGGD
ANVLSRGILGDAAGEPVVISPLYKQRIRLRDGCQVENGEPAVRTWPVKIENGKVWVGSDA
LVMRAEAS